L-Selectin Antikörper

Produktnummer ABIN2689360

Produktdetails anti-L-Selectin Antikörper

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Marke: BD Pharmingen™

Produktmerkmale: The MEL-14 antibody reacts with CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymphocytes and neutrophils upon cell activation, metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of CD62L expression on spleen lymphocytes. BALB/c splenocytes were simultaneously stained with purified MEL-14 (right panel) and PE-conjugated anti-mouse CD3e 145-2C11 (Cat. No. 553063/553064, both panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD62L - Purified - Clone MEL-14 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H/eb mouse B lymphoma 38C-13

Klon: MEL

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-L-Selectin Antikörper (ABIN2689360)

Lanzavecchia, Sallusto: "Dynamics of T lymphocyte responses: intermediates, effectors, and memory cells." in: Science (New York, N.Y.), Vol. 290, Issue 5489, pp. 92-7, (2000) (PubMed).

Cerwenka, Carter, Reome, Swain, Dutton: "In vivo persistence of CD8 polarized T cell subsets producing type 1 or type 2 cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 1, pp. 97-105, (1998) (PubMed).

Peschon, Slack, Reddy, Stocking, Sunnarborg, Lee, Russell, Castner, Johnson, Fitzner, Boyce, Nelson, Kozlosky, Wolfson, Rauch, Cerretti, Paxton, March, Black: "An essential role for ectodomain shedding in mammalian development." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1281-4, (1998) (PubMed).

Seibold, Seibold-Schmid, Cong, Shu, McCabe, Weaver, Elson: "Regional differences in L-selectin expression in murine intestinal lymphocytes." in: Gastroenterology, Vol. 114, Issue 5, pp. 965-74, (1998) (PubMed).

Yang, Mizuno, Hellström, Chen: "B7-negative versus B7-positive P815 tumor: differential requirements for priming of an antitumor immune response in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 851-8, (1997) (PubMed).

Vestweber: "Ligand-specificity of the selectins." in: Journal of cellular biochemistry, Vol. 61, Issue 4, pp. 585-91, (1996) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Pizcueta, Luscinskas: "Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo." in: The American journal of pathology, Vol. 145, Issue 2, pp. 461-9, (1994) (PubMed).

Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, (1994) (PubMed).

Iwabuchi, Ohgama, Ogasawara, Iwabuchi, Negishi, Good, Onoé: "Distribution of MEL-14+ cells in various lymphoid tissues." in: Immunobiology, Vol. 182, Issue 2, pp. 161-73, (1991) (PubMed).

Siegelman, Cheng, Weissman, Wakeland: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, (1990) (PubMed).

Jung, Gallatin, Weissman, Dailey: "Down-regulation of homing receptors after T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4110-7, (1989) (PubMed).

Kishimoto, Jutila, Berg, Butcher: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, (1989) (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, (1987) (PubMed).

Shortman, Wilson, Van Ewijk, Scollay: "Phenotype and localization of thymocytes expressing the homing receptor-associated antigen MEL-14: arguments for the view that most mature thymocytes are located in the medulla." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 342-51, (1987) (PubMed).

Reichert, Weissman, Butcher: "Dual immunofluorescence studies of cortisone-induced thymic involution: evidence for a major cortical component to cortisone-resistant thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 10, pp. 3529-34, (1986) (PubMed).

Reichert, Jerabek, Gallatin, Butcher, Weissman: "Ontogeny of lymphocyte homing receptor expression in the mouse thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 10, pp. 3535-42, (1986) (PubMed).

Reichert, Weissman, Butcher: "Phenotypic analysis of thymocytes that express homing receptors for peripheral lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 10, pp. 3521-8, (1986) (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, (1983) (PubMed).

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: CD62L (SELL Produkte)

Synonyme: CD62L antikoerper, LAM1 antikoerper, LECAM1 antikoerper, LEU8 antikoerper, LNHR antikoerper, LSEL antikoerper, LYAM1 antikoerper, PLNHR antikoerper, TQ1 antikoerper, A.11 antikoerper, L-selectin antikoerper, LECAM-1 antikoerper, SELE antikoerper, AI528707 antikoerper, Lnhr antikoerper, Ly-22 antikoerper, Ly-m22 antikoerper, Lyam-1 antikoerper, Lyam1 antikoerper, selectin L antikoerper, selectin, lymphocyte antikoerper, SELL antikoerper, Sell antikoerper

Substanzklasse: Chemical

Hintergrund: Synonyms: L-selectin, LECAM-1, Ly-22