CD51 Antikörper (Biotin)

Produktnummer ABIN2689325

Produktdetails anti-CD51 Antikörper (Biotin)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD51 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The rat anti-mouse CD51 (clone RMV-7) antibody reacts with the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). It has been reported to be expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 has reportedly been found to be undetectable on mouse platelets using either antibody clones H9.2B8 or RMV-7. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The rat anti-mouse (clone RMV-7) mAb has been reported to block LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 clone reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD51 on mouse bone marrow myeloid cells. BALB/c bone marrow leukocytes were stained with biotin rat anti-mouse CD51 (clone RMV-7) (right panel), followed by streptavidin-PE (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™ instrument . Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel).

BD Pharmingen™ Biotin Rat Anti-Mouse CD51 - Biotin - Clone RMV-7 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse (BALB/c) IL-2-activated killer (LAK) Cells

Klon: RMV

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD51 Antikörper (Biotin) (ABIN2689325)

Nakamura, Pilkington, Lakkakorpi, Lipfert, Sims, Dixon, Rodan, Duong: "Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone." in: Journal of cell science, Vol. 112 ( Pt 22), pp. 3985-93, (2000) (PubMed).

Bader, Rayburn, Crowley, Hynes: "Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins." in: Cell, Vol. 95, Issue 4, pp. 507-19, (1998) (PubMed).

Frieser, Hallmann, Johansson, Vestweber, Goodman, Sorokin: "Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins." in: European journal of immunology, Vol. 26, Issue 12, pp. 3127-36, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Narumiya, Abe, Kita, Miyake, Nakajima, Watanabe, Oka, Sugiyama, Yagita, Okumura: "Pre-B cells adhere to fibronectin via interactions of integrin alpha 5/alpha V with RGDS as well as of integrin alpha 4 with two distinct V region sequences at its different binding sites." in: International immunology, Vol. 6, Issue 1, pp. 139-47, (1994) (PubMed).

Moulder, Roberts, Shevach, Coligan: "The mouse vitronectin receptor is a T cell activation antigen." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 343-7, (1991) (PubMed).

Details zu CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Andere Bezeichnung: CD51 (ITGAV Produkte)

Synonyme: 1110004F14Rik antikoerper, 2610028E01Rik antikoerper, CD51 antikoerper, D430040G12Rik antikoerper, MSK8 antikoerper, VNRA antikoerper, VTNR antikoerper, Cd51 antikoerper, integrin subunit alpha V antikoerper, integrin alpha V antikoerper, ITGAV antikoerper, Itgav antikoerper

Hintergrund: Synonyms: Integrin αV chain

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex