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ITGA2 Antikörper (Biotin)

ITGA2 Reaktivität: Maus FACS, IHC (p), IHC (fro) Wirt: Ratte Monoclonal DX5 Biotin
Produktnummer ABIN2689298
  • Target Alle ITGA2 Antikörper anzeigen
    ITGA2 (Integrin alpha 2 (ITGA2))
    Reaktivität
    • 130
    • 72
    • 21
    • 12
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 82
    • 47
    • 29
    • 7
    • 7
    Ratte
    Klonalität
    • 100
    • 75
    Monoklonal
    Konjugat
    • 71
    • 24
    • 18
    • 16
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ITGA2 Antikörper ist konjugiert mit Biotin
    Applikation
    • 82
    • 67
    • 44
    • 42
    • 19
    • 17
    • 15
    • 12
    • 11
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Marke
    BD Pharmingen™
    Produktmerkmale
    The rat anti-mouse CD49b monoclonal antibody (clone DX5) specifically binds to the integrin α2 chain (CD49b). CD49b is a 150 kDa transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1) to form the integrin α2β1 complex known as VLA-2. The rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most mouse strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not NOD). The DX5 antibody also recognizes platelets that express high levels of CD49b. Multiparameter flow cytometric analysis has demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody (clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ cell subsets are found, especially among the CD3-positive cell population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody (clone DX5) when cultured in the presence of recombinant human IL-2. The resulting DX5-negative cells have weakened cytotoxic activity when compared to the remaining DX5+ cells. This indicates that the DX5 antibody distinguishes functional subsets of NK cells. No activation or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed. Staining of splenic NK cells with this antibody reportedly can be blocked by hamster anti-mouse CD49b antibody (clone HMα2). Detection of NK cells with monoclonal antibodies. Freshly isolated splenocytes from a C57BL/6 mouse were simultaneously stained with FITC Mouse anti-Mouse NK-1.1 antibody (Cat. No. 553164, both panels) and Biotin Rat anti-Mouse CD49b antibody (clone DX5) (right panel) followed by PE-Streptavidin (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™ instrument .

    BD Pharmingen™ Biotin Rat Anti-Mouse CD49b - Biotin - Clone DX5 - Isotype Rat IgM, κ - Reactivity Ms - 0.5 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2
    Klon
    DX5
    Isotyp
    IgM kappa
    Top Product
    Discover our top product ITGA2 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Arase, Saito, Phillips, Lanier: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).

    Ortaldo, Winkler-Pickett, Mason, Mason: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).

    Moore, von Freeden-Jeffry, Murray, Zlotnik: "Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2366-73, (1996) (PubMed).

  • Target
    ITGA2 (Integrin alpha 2 (ITGA2))
    Andere Bezeichnung
    CD49b (ITGA2 Produkte)
    Synonyme
    BR antikoerper, CD49B antikoerper, GPIa antikoerper, HPA-5 antikoerper, VLA-2 antikoerper, VLAA2 antikoerper, DX5 antikoerper, integrin subunit alpha 2 antikoerper, integrin alpha 2 antikoerper, ITGA2 antikoerper, Itga2 antikoerper
    Hintergrund
    Synonyms: itga2, Integrin alpha-2, DX5, Pan NK cell marker, VLAA2, VLA-2 alpha chain
    Pathways
    CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
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