CD35 Antikörper (Biotin)

Produktnummer ABIN2689207

Produktdetails anti-CD35 Antikörper (Biotin)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD35 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: The 8C12 antibody recognizes an epitope present on the 190- kDa complement receptor protein, originally designated CR1 (CD35), but not the 145-150- kDa CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene. Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively. The epitope recognized by 8C12 mAb is only present on CD35/CD21b. Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages. The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes, to block the complement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon in vivo application, inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2[null] mice display impaired humoral immune responses in vivo. The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6 (Cat. no. 553817), and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.

BD Pharmingen™ Biotin Rat Anti-Mouse CD35 - Biotin - Clone 8C12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified mouse CR1

Klon: 8C12

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD35 Antikörper (Biotin) (ABIN2689207)

Fischer, Goerg, Shen, Prodeus, Goodnow, Kelsoe, Carroll: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, (1998) (PubMed).

Fu, Huang, Wang, Chaplin: "B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1009-18, (1998) (PubMed).

Hu, Martin, Weis, Weis: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, (1997) (PubMed).

Ahearn, Fischer, Croix, Goerg, Ma, Xia, Zhou, Howard, Rothstein, Carroll: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, (1996) (PubMed).

Molina, Holers, Li, Fung, Mariathasan, Goellner, Strauss-Schoenberger, Karr, Chaplin: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, (1996) (PubMed).

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, (1993) (PubMed).

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, (1993) (PubMed).

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, (1991) (PubMed).

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, (1990) (PubMed).

Kurtz, OToole, Christensen, Weis: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, (1990) (PubMed).

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CD35 (CR1 Produkte)

Synonyme: C3BR antikoerper, C4BR antikoerper, CD35 antikoerper, KN antikoerper, C3DR antikoerper, CD21 antikoerper, Cr-1 antikoerper, Cr-2 antikoerper, Cr1 antikoerper, CR1-L antikoerper, CR2 antikoerper, CR1 antikoerper, complement C3b/C4b receptor 1 (Knops blood group) antikoerper, complement receptor 2 antikoerper, complement component (3d/Epstein Barr virus) receptor 2 antikoerper, complement receptor type 1 antikoerper, CR1 antikoerper, Cr2 antikoerper, CR2 antikoerper, LOC718120 antikoerper

Hintergrund: Synonyms: CR1, CD21b

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity