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CD27 Antikörper (Biotin)

CD27 Reaktivität: Maus, Ratte FACS Wirt: Armenischer Hamster Monoclonal LG-3A10 Biotin
Produktnummer ABIN2689133
  • Target Alle CD27 Antikörper anzeigen
    CD27 (CD27 Molecule (CD27))
    Reaktivität
    • 189
    • 60
    • 45
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Maus, Ratte
    Wirt
    • 104
    • 90
    • 15
    • 10
    • 3
    • 1
    Armenischer Hamster
    Klonalität
    • 133
    • 88
    • 2
    Monoklonal
    Konjugat
    • 74
    • 23
    • 21
    • 14
    • 11
    • 7
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD27 Antikörper ist konjugiert mit Biotin
    Applikation
    • 145
    • 84
    • 51
    • 35
    • 33
    • 20
    • 17
    • 13
    • 13
    • 13
    • 11
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Marke
    BD Pharmingen™
    Produktmerkmale
    The LG.3A10 antibody reacts with CD27, a lymphocyte-restricted member of the Tumor Necrosis Factor Receptor family which binds to CD70. The CD27 Molecule is a 45- kDa transmembrane glycoprotein which is constitutively expressed by lymphocytes of the T lineage: virtually all thymocytes and over 90 % of peripheral T cells bearing both αβ and γδ T-cell receptors. CD27 cooperates with the pre-TCR in mediating thymocyte differentiation and expansion. In addition, one to ten percent of mature peripheral B cells express CD27, and CD27's role in the differentiation of human plasma cells has been studied. Mouse NK cells, freshly isolated and IL-2-activated, also express CD27. In the bone marrow, CD27 is found on a progenitor population which provides short-term hematopoietic reconstitution. Cells of the myeloid lineage do not express CD27. Cross-linked LG.3A10 mAb has been reported to amplify the proliferative response of purified T lymphocytes to suboptimal mitogenic stimulation1 and to enhance NK-cell proliferation and IFN-γ production. In contrast, non-cross-linked LG.3A10 mAb inhibits CD3-induced pre-T cell development by interfering with the receptor-ligand interaction. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with a similar population of rat leukocytes. Expression of CD27 on mouse splenocytes. C57BL/6 splenocytes were stained simultaneously with PE-conjugated anti-mouse CD3e mAb 500A2 (Cat. No. 553240) and biotinylated mAb LG.3A10 (right panel), followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

    BD Pharmingen™ Biotin Hamster Anti-Mouse CD27 - Biotin - Clone LG.3A10 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms, Rat - 0.1 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA
    Klon
    LG-3A10
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product CD27 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Agematsu, Hokibara, Nagumo, Shinozaki, Yamada, Komiyama: "Plasma cell generation from B-lymphocytes via CD27/CD70 interaction." in: Leukemia & lymphoma, Vol. 35, Issue 3-4, pp. 219-25, (2000) (PubMed).

    Takeda, Oshima, Hayakawa, Akiba, Atsuta, Kobata, Kobayashi, Ito, Yagita, Okumura: "CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).

    Wiesmann, Phillips, Mojica, Pierce, Searles, Spangrude, Lemischka: "Expression of CD27 on murine hematopoietic stem and progenitor cells." in: Immunity, Vol. 12, Issue 2, pp. 193-9, (2000) (PubMed).

    Gravestein, van Ewijk, Ossendorp, Borst: "CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 675-85, (1996) (PubMed).

    Gravestein, Nieland, Kruisbeek, Borst: "Novel mAbs reveal potent co-stimulatory activity of murine CD27." in: International immunology, Vol. 7, Issue 4, pp. 551-7, (1995) (PubMed).

    Gravestein, Blom, Nolten, de Vries, van der Horst, Ossendorp, Borst, Loenen: "Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family." in: European journal of immunology, Vol. 23, Issue 4, pp. 943-50, (1993) (PubMed).

  • Target
    CD27 (CD27 Molecule (CD27))
    Andere Bezeichnung
    CD27 (CD27 Produkte)
    Synonyme
    TNFRSF7 antikoerper, S152 antikoerper, Tnfrsf7 antikoerper, Tp55 antikoerper, S152. LPFS2 antikoerper, T14 antikoerper, CD27 molecule antikoerper, CD27 antigen antikoerper, CD27 antikoerper, Cd27 antikoerper
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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