CD25 Antikörper

Produktnummer ABIN2689118

Produktdetails anti-CD25 Antikörper

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Reagent (BR)

Marke: BD Pharmingen™

Produktmerkmale: The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25. Use of this product can fall under one or more claims of the following patents licensed to Becton, Dickinson and Company: US Patent Nos. 5,445,939, 5,656,446, 5,843,689, European Patent No. 319,543, Canadian Patent No. 1,296,622, Australian Patent No. 615,880, and Japanese Patent No. 2,769,156. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD25 - Purified - Clone PC61 - Isotype Rat IgG1, λ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1

Klon: PC61

Isotyp: IgG1 lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD25 Antikörper (ABIN2689118)

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, (2000) (PubMed).

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, (1995) (PubMed).

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, (1995) (PubMed).

Godfrey, Zlotnik: "Control points in early T-cell development." in: Immunology today, Vol. 14, Issue 11, pp. 547-53, (1994) (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, (1990) (PubMed).

Pollard, Lipscomb: "Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 159-67, (1990) (PubMed).

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, (1987) (PubMed).

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, (1985) (PubMed).

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, (1985) (PubMed).

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, (1985) (PubMed).

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25 (IL2RA Produkte)

Synonyme: CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund: Synonyms: IL-2 Receptor α chain, p55

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation