IL-10RA Antikörper

Produktnummer ABIN2689090

Produktdetails anti-IL-10RA Antikörper

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-10RA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R. Mouse B220+ splenocytes were stained with purified 1B1.3a (0.25 μg, Cat. No. 559912) followed by biotinylated polyclonal goat anti-rat Ig (0.25 μg, Cat. No. 554014) and Streptavidin-PE (0.015 μg, Cat. No. 554061) in a three layer staining protocol to amplify immunofluorescent signals (left panel). In addition, cells in buffer containing sodium azide were pre-incubated on ice with recombinant mouse IL-10 (0.25 μg, Cat. No. 554585) prior to staining with 1B1.3a to determine the blocking effect of bound IL-10 protein on staining with 1B1.3a (right panel). Staining with the purified 1B1.3a antibody (filled histograms) is compared to the staining obtained with the secondary antibody alone (open histograms). Histograms in both panels are gated on the lymphocyte population as defined by light scattering characteristics and were also gated on the cell subset stained with FITC-conjugated rat-anti-mouse B220 (0.06 μg, Cat. No. 553088).

BD Pharmingen™ Purified Rat Anti-Mouse CD210 - Purified - Clone 1B1.3a - Isotype Rat IgG1, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified recombinant ligand-binding domain of mIL-10R

Klon: 1B1-3a

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IL-10RA Antikörper (ABIN2689090)

OFarrell, Liu, Moore, Mui: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).

Details zu IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Andere Bezeichnung: CD210 (IL10RA Produkte)

Synonyme: IL10R1 antikoerper, IL10RA antikoerper, CD210 antikoerper, CD210a antikoerper, CDW210A antikoerper, HIL-10R antikoerper, IL-10R1 antikoerper, IL10R antikoerper, AW553859 antikoerper, CDw210 antikoerper, CDw210a antikoerper, Il10r antikoerper, mIL-10R antikoerper, interleukin 10 receptor subunit alpha antikoerper, interleukin 10 receptor, alpha antikoerper, IL10RA antikoerper, Il10ra antikoerper

Hintergrund: Synonyms: IL-10 Receptor

Pathways: Growth Factor Binding