CCR7 Antikörper (Biotin)

Produktnummer ABIN2689061

Produktdetails anti-CCR7 Antikörper (Biotin)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CCR7 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The monoclonal antibody 3D12 reacts with the human CC chemokine receptor, CCR7. CCR7 (previously known as BLR-2, EBI-1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3β/Exodus 3/ELC/ CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL21. It has been shown that CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. CCR7 mRNA was also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17q12. The immunogen used to generate 3D12 hybridoma was the N-terminus as well as parts of the second extracellular loop of human CCR7 protein. The monoclonal antibody 3D12 recognizes an epitode mapping to the N-terminus of human CCR7. Detection of CCR7 expression on CD4 and CD8-positive human peripheral lymphocytes by biotinylated anti-human CCR7 antibody 3D12. Human PBMC were stained with 20 μL/test of biotinylated 3D12 and anti-human CD45RA-FITC (Cat. No. 555488). The data shown are derived from the CD4-positive (based on staining with anti-human CD4-APC, Cat. No. 555349, left panel) and CD8-positive (based on staining with anti-human CD8-APC, Cat. No. 555369, right panel) lymphocyte gated populations and displayed as bivariate dot plots.

BD Pharmingen™ Biotin Rat Anti-Human CD197 (CCR7) - Biotin - Clone 3D12 - Isotype Rat IgG2a, κ - Reactivity Hu - 100 Tests

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CCR7 protein

Klon: 3D12

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CCR7 Antikörper (Biotin) (ABIN2689061)

Lipp, Burgstahler, Müller, Pevzner, Kremmer, Wolf, Förster: "Functional organization of secondary lymphoid organs by the chemokine system." in: Current topics in microbiology and immunology, Vol. 251, pp. 173-9, (2000) (PubMed).

Sallusto, Lenig, Förster, Lipp, Lanzavecchia: "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions." in: Nature, Vol. 401, Issue 6754, pp. 708-12, (1999) (PubMed).

Kim, Pelus, White, Broxmeyer: "Macrophage-inflammatory protein-3 beta/EBI1-ligand chemokine/CK beta-11, a CC chemokine, is a chemoattractant with a specificity for macrophage progenitors among myeloid progenitor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 5, pp. 2580-5, (1998) (PubMed).

Yanagihara, Komura, Nagafune, Watarai, Yamaguchi: "EBI1/CCR7 is a new member of dendritic cell chemokine receptor that is up-regulated upon maturation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 6, pp. 3096-102, (1998) (PubMed).

Yoshida, Nagira, Imai, Baba, Takagi, Tabira, Akagi, Nomiyama, Yoshie: "EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC." in: International immunology, Vol. 10, Issue 7, pp. 901-10, (1998) (PubMed).

Yoshida, Nagira, Kitaura, Imagawa, Imai, Yoshie: "Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7." in: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 7118-22, (1998) (PubMed).

Yoshida, Imai, Hieshima, Kusuda, Baba, Kitaura, Nishimura, Kakizaki, Nomiyama, Yoshie: "Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13803-9, (1997) (PubMed).

Burgstahler, Kempkes, Steube, Lipp: "Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr virus nuclear antigen 2." in: Biochemical and biophysical research communications, Vol. 215, Issue 2, pp. 737-43, (1995) (PubMed).

Schweickart, Raport, Godiska, Byers, Eddy, Shows, Gray: "Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2." in: Genomics, Vol. 23, Issue 3, pp. 643-50, (1995) (PubMed).

Birkenbach, Josefsen, Yalamanchili, Lenoir, Kieff: "Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors." in: Journal of virology, Vol. 67, Issue 4, pp. 2209-20, (1993) (PubMed).

Details zu CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Andere Bezeichnung: CD197 CCR7 (CCR7 Produkte)

Synonyme: zgc:165629 antikoerper, ccr7-a antikoerper, CD197 antikoerper, Cdw197 antikoerper, Cmkbr7 antikoerper, EBI1 antikoerper, Ebi1h antikoerper, BLR2 antikoerper, CDw197 antikoerper, CMKBR7 antikoerper, chemokine (C-C motif) receptor 7 antikoerper, C-C motif chemokine receptor 7 antikoerper, C-C motif chemokine receptor 7 L homeolog antikoerper, ccr7 antikoerper, CCR7 antikoerper, ccr7.L antikoerper, Ccr7 antikoerper

Hintergrund: Synonyms: CCR7, BLR-2, EBI-1, CMKBR7

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process