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CD180 Antikörper (Biotin)

CD180 Reaktivität: Maus FACS Wirt: Ratte Monoclonal RP-14 Biotin
Produktnummer ABIN2689038
  • Target Alle CD180 Antikörper anzeigen
    CD180 (CD180 Molecule (CD180))
    Reaktivität
    • 21
    • 12
    • 3
    • 1
    Maus
    Wirt
    • 12
    • 9
    • 5
    Ratte
    Klonalität
    • 14
    • 12
    Monoklonal
    Konjugat
    • 19
    • 4
    • 2
    • 1
    Dieser CD180 Antikörper ist konjugiert mit Biotin
    Applikation
    • 15
    • 13
    • 8
    • 8
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    Flow Cytometry (FACS)
    Marke
    BD Pharmingen™
    Produktmerkmale
    The RP/14 antibody reacts with CD180, the "radioprotective" 105 kDa (RP105) antigen expressed on mature B lymphocytes. Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines. These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses. Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface. Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes. Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c.xid, C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c.xid, C57BL/6.xid, CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14. Two-color analysis of CD180 expression on splenic B lymphocytes. BALB/c splenocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553928, left panel) or biotinylated mAb RP/14 (right panel) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by Streptavidin-PE (Cat. No. 554061) and FITC-conjugated anti-mouse CD45R/B220 mAb RA3-6B2 (Cat. No. 553087/553088). Flow cytometry was performed on a FACSCalibur™ .

    BD Pharmingen™ Biotin Rat Anti-Mouse CD180 - Biotin - Clone RP/14 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    BALB/c mouse splenocytes
    Klon
    RP-14
    Isotyp
    IgG2a kappa
    Top Product
    Discover our top product CD180 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Ogata, Su, Miyake, Nagai, Akashi, Mecklenbräuker, Rajewsky, Kimoto, Tarakhovsky: "The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells." in: The Journal of experimental medicine, Vol. 192, Issue 1, pp. 23-9, (2000) (PubMed).

    Corcoran, Metcalf: "IL-5 and Rp105 signaling defects in B cells from commonly used 129 mouse substrains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 5836-42, (1999) (PubMed).

    Chan, Mecklenbräuker, Su, Texido, Leitges, Carsetti, Lowell, Rajewsky, Miyake, Tarakhovsky: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." in: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, (1998) (PubMed).

    Miyake, Shimazu, Kondo, Niki, Akashi, Ogata, Yamashita, Miura, Kimoto: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, (1998) (PubMed).

    Yamashita, Miyake, Miura, Kaneko, Yagita, Suda, Nagata, Nomura, Sakaguchi, Kimoto: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, (1996) (PubMed).

    Miyake, Yamashita, Ogata, Sudo, Kimoto: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, (1995) (PubMed).

    Miyake, Yamashita, Hitoshi, Takatsu, Kimoto: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, (1994) (PubMed).

  • Target
    CD180 (CD180 Molecule (CD180))
    Andere Bezeichnung
    CD180 (CD180 Produkte)
    Synonyme
    Ly78 antikoerper, CD180 antikoerper, LY64 antikoerper, RP105 antikoerper, F630107B15 antikoerper, CD180 molecule antikoerper, CD180 antigen antikoerper, Cd180 antikoerper, CD180 antikoerper
    Hintergrund
    Synonyms: RP105
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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