IGLL1 Antikörper (Biotin)

Produktnummer ABIN2689029

Produktdetails anti-IGLL1 Antikörper (Biotin)

Target: IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IGLL1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b (λ5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with λ5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or λ5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37 °C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface λ5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes. Two-color analysis of CD179b (?5) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgMb mAb AF6-78 (Cat. no. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37 °C to enhance surrogate light chain expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553928, left panel) or biotinylated mAb LM34 (right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142), followed by Streptavidin-APC (Cat. no. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb 9 RA3-6B2 (Cat. no. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD179b - Biotin - Clone LM34 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified soluble complex of recombinant mu IgH, lambda5, and V[preB]

Klon: LM34

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IGLL1 Antikörper (Biotin) (ABIN2689029)

Shimizu, Mundt, Licence, Melchers, Mårtensson: "VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6286-93, (2002) (PubMed).

Ohnishi, Shimizu, Karasuyama, Melchers: "The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain." in: The Journal of biological chemistry, Vol. 275, Issue 40, pp. 31134-44, (2000) (PubMed).

Mårtensson, Ceredig: "Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development." in: Immunology, Vol. 101, Issue 4, pp. 435-41, (2000) (PubMed).

Melchers, ten Boekel, Seidl, Kong, Yamagami, Onishi, Shimizu, Rolink, Andersson: "Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells." in: Immunological reviews, Vol. 175, pp. 33-46, (2000) (PubMed).

Meffre, Papavasiliou, Cohen, de Bouteiller, Bell, Karasuyama, Schiff, Banchereau, Liu, Nussenzweig: "Antigen receptor engagement turns off the V(D)J recombination machinery in human tonsil B cells." in: The Journal of experimental medicine, Vol. 188, Issue 4, pp. 765-72, (1998) (PubMed).

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, (1995) (PubMed).

Winkler, Rolink, Melchers, Karasuyama: "Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface." in: European journal of immunology, Vol. 25, Issue 2, pp. 446-50, (1995) (PubMed).

Karasuyama, Rolink, Shinkai, Young, Alt, Melchers: "The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice." in: Cell, Vol. 77, Issue 1, pp. 133-43, (1994) (PubMed).

Karasuyama, Rolink, Melchers: "A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 469-78, (1993) (PubMed).

Details zu IGLL1

Target: IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))

Andere Bezeichnung: CD179b (IGLL1 Produkte)

Synonyme: 14.1 antikoerper, AGM2 antikoerper, CD179b antikoerper, IGL1 antikoerper, IGL5 antikoerper, IGLJ14.1 antikoerper, IGLL antikoerper, IGO antikoerper, IGVPB antikoerper, VPREB2 antikoerper, IGLV antikoerper, IGLL1 antikoerper, MGC151892 antikoerper, Igll1 antikoerper, BB139905 antikoerper, Igl-5 antikoerper, Igll antikoerper, Lambda5 antikoerper, immunoglobulin lambda like polypeptide 1 antikoerper, immunoglobulin lambda-like polypeptide 1 antikoerper, IGLL1 antikoerper, Igll1 antikoerper

Hintergrund: Synonyms: λ5

Pathways: Regulation of Cell Size