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FASL Antikörper (Biotin)

FASL Reaktivität: Human WB, FACS Wirt: Maus Monoclonal NOK Biotin
Produktnummer ABIN2689027
  • Target Alle FASL Antikörper anzeigen
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    Reaktivität
    • 103
    • 52
    • 35
    • 18
    • 6
    • 5
    • 3
    • 2
    • 1
    Human
    Wirt
    • 69
    • 35
    • 10
    • 5
    • 4
    • 3
    • 1
    Maus
    Klonalität
    • 76
    • 51
    Monoklonal
    Konjugat
    • 74
    • 13
    • 10
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FASL Antikörper ist konjugiert mit Biotin
    Applikation
    • 83
    • 63
    • 28
    • 25
    • 23
    • 17
    • 13
    • 13
    • 11
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Marke
    BD Pharmingen™
    Produktmerkmale
    Fas (CD95, APO-1) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or by Fas ligand (FasL, CD178). Fas belongs to the TNF (Tumor Necrosis Factor)/NGF (Nerve Growth Factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. CD178 (FasL), a member of the TNF cytokine family, induces apoptosis by binding to Fas, its cell-surface receptor. FasL may exist as either membrane bound or soluble forms and is expressed by activated T and NK cells. FasL may also be constitutively expressed in some immunologically privileged sites, e.g., eye and testis. Fas and FasL play an important role in the induction of apoptosis, and thus regulate a variety of immunological responses. The NOK-1 antibody clone has been reported to recognize human FasL, recognizing both the membrane bound (FasL) and soluble (sFasL) forms. It is reported that the epitope for NOK-1 has been mapped to the COOH-terminus of FasL, at the region implicated in Fas binding. FasL and sFasL have been reported to migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in the literature. The NOK-1 antibody clone is not recommended for the western blot application. Flow cytometric analysis of human Fas Ligand (FasL). The mouse T cell lymphoma cell line, L5187Y was transfected with human FasL cDNA and treated with a metalloproteinase inhibitor, KB8301 (left panel). KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. As expected, FasL was not detected in the parental L5187Y cells after KB8301 treatment (right panel). The data was generated using a 2-step procedure with either biotin-labeled, isotype-matched control antibody (dashed line) or biotin-labeled (NOK-1, Cat. No. 556373) (solid line) followed by Streptavidin-PE.

    BD Pharmingen™ Biotin Mouse Anti-Human CD178 - Biotin - Clone NOK-1 - Isotype Mouse IgG1 - Reactivity Hu - 0.1 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse T lymphoma cells (L5178Y) expressing human FasL
    Klon
    NOK
    Isotyp
    IgG1
    Top Product
    Discover our top product FASL Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Orlinick, Elkon, Chao: "Separate domains of the human fas ligand dictate self-association and receptor binding." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32221-9, (1998) (PubMed).

    Griffith, Ferguson: "The role of FasL-induced apoptosis in immune privilege." in: Immunology today, Vol. 18, Issue 5, pp. 240-4, (1997) (PubMed).

    Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).

    Lynch, Ramsdell, Alderson: "Fas and FasL in the homeostatic regulation of immune responses." in: Immunology today, Vol. 16, Issue 12, pp. 569-74, (1996) (PubMed).

    Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

    Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

  • Target
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    Andere Bezeichnung
    CD178 (FASL Produkte)
    Synonyme
    ALPS1B antikoerper, APT1LG1 antikoerper, APTL antikoerper, CD178 antikoerper, CD95-L antikoerper, CD95L antikoerper, FASL antikoerper, TNFSF6 antikoerper, fasl antikoerper, Fas-L antikoerper, Faslg antikoerper, Tnfsf6 antikoerper, gld antikoerper, Apt1Lg1 antikoerper, Fasl antikoerper, FASLG antikoerper, zgc:162027 antikoerper, Fas ligand antikoerper, Fas ligand L homeolog antikoerper, Fas ligand (TNF superfamily, member 6) antikoerper, FASLG antikoerper, faslg.L antikoerper, Fasl antikoerper, Faslg antikoerper, faslg antikoerper
    Hintergrund
    Synonyms: Fas Ligand, CD95 Ligand
    Pathways
    Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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