Fas (CD95, APO-1) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or by Fas ligand (FasL, CD178). Fas belongs to the TNF (Tumor Necrosis Factor)/NGF (Nerve Growth Factor) receptor family, and is expressed in various tissues and cells including the thymus, liver, ovary and lung. CD178 (FasL), a member of the TNF cytokine family, induces apoptosis by binding to Fas, its cell-surface receptor. FasL may exist as either membrane bound or soluble forms and is expressed by activated T and NK cells. FasL may also be constitutively expressed in some immunologically privileged sites, e.g., eye and testis. Fas and FasL play an important role in the induction of apoptosis, and thus regulate a variety of immunological responses. The NOK-1 antibody clone has been reported to recognize human FasL, recognizing both the membrane bound (FasL) and soluble (sFasL) forms. It is reported that the epitope for NOK-1 has been mapped to the COOH-terminus of FasL, at the region implicated in Fas binding. FasL and sFasL have been reported to migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in the literature. The NOK-1 antibody clone is not recommended for the western blot application. Flow cytometric analysis of human Fas Ligand (FasL). The mouse T cell lymphoma cell line, L5187Y was transfected with human FasL cDNA and treated with a metalloproteinase inhibitor, KB8301 (left panel). KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. As expected, FasL was not detected in the parental L5187Y cells after KB8301 treatment (right panel). The data was generated using a 2-step procedure with either biotin-labeled, isotype-matched control antibody (dashed line) or biotin-labeled (NOK-1, Cat. No. 556373) (solid line) followed by Streptavidin-PE.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Target
FASL
(Fas Ligand (TNF Superfamily, Member 6) (FASL))