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FASL Antikörper

FASL Reaktivität: Human WB, FACS, ELISA, IP, Neut Wirt: Maus Monoclonal NOK unconjugated
Produktnummer ABIN2689020
  • Target Alle FASL Antikörper anzeigen
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    Reaktivität
    • 103
    • 52
    • 35
    • 18
    • 6
    • 5
    • 3
    • 2
    • 1
    Human
    Wirt
    • 69
    • 35
    • 10
    • 5
    • 4
    • 3
    • 1
    Maus
    Klonalität
    • 76
    • 51
    Monoklonal
    Konjugat
    • 73
    • 14
    • 10
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FASL Antikörper ist unkonjugiert
    Applikation
    • 83
    • 63
    • 27
    • 24
    • 23
    • 17
    • 13
    • 13
    • 11
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Neutralization (Neut)
    Marke
    BD Pharmingen™
    Produktmerkmale
    Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung. FasL is a 40 kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. NOK-2 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al. Immunoprecipitation of Fas Ligand (FasL). T lymphoma L5187Y cells were transfected with human FasL cDNA and treated or not treated with metalloprotease inhibitor KB8301. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in S-labeled cells treated with inhibitor KB8301 (lane 1), but not in untreated cells (lane 3). Isotype (negative) controls are shown in lanes 2 and 4. Purified NOK-2 (Cat. No. 556376) or mouse IgG2a (Cat. No. 553454) was used for immunoprecipitations.

    BD Pharmingen™ Purified Mouse Anti-Human CD178 - Purified - Clone NOK-2 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL
    Klon
    NOK
    Isotyp
    IgG2a
    Top Product
    Discover our top product FASL Primärantikörper
  • Applikationshinweise
    Flow Cytometry : Another clone, NOK-1, (556372, 556373) are recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1. Western Blot: Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 is suggested for the western blot analysis.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).

    Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

    Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

  • Target
    FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
    Andere Bezeichnung
    CD178 (FASL Produkte)
    Synonyme
    ALPS1B antikoerper, APT1LG1 antikoerper, APTL antikoerper, CD178 antikoerper, CD95-L antikoerper, CD95L antikoerper, FASL antikoerper, TNFSF6 antikoerper, fasl antikoerper, Fas-L antikoerper, Faslg antikoerper, Tnfsf6 antikoerper, gld antikoerper, Apt1Lg1 antikoerper, Fasl antikoerper, FASLG antikoerper, zgc:162027 antikoerper, Fas ligand antikoerper, Fas ligand L homeolog antikoerper, Fas ligand (TNF superfamily, member 6) antikoerper, FASLG antikoerper, faslg.L antikoerper, Fasl antikoerper, Faslg antikoerper, faslg antikoerper
    Hintergrund
    Synonyms: Fas Ligand, CD95 Ligand
    Molekulargewicht
    40, 26 kDa
    Pathways
    Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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