IL-6 Receptor Antikörper

Produktnummer ABIN2688945

Produktdetails anti-IL-6 Receptor Antikörper

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-6 Receptor Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The M5 antibody reacts with human CD126 which is also known as the a subunit of the human IL-6 Receptor (IL-6Rα). The human IL-6Rais an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor. The IL-6Rα subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3). The IL-6Rα chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6Rα complex resulting in the formation of a high affinity complex. The IL-6R ßchain mediates signal transduction. IL-6Rα's are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells. IL-6Rα exists in soluble form in human serum. The serum levels of soluble IL-6Rα appear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV. The immunogen used to generate the M5 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6Rα). Expression of cell surface IL-6R by human PBMC. Human PBMC isolated by density centrifugation (Ficoll-Paque™) were blocked with normal polyclonal human IgG and stained with M5 (0.5 μg, Cat No. 551462) followed by biotinylated rat anti-mouse IgG1 (0.06 μg, Cat . No. 553441) and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the M5 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). Histogram in figure is gated on the CD19 lymphocytes. Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors (for example see Browning et al).

BD Pharmingen™ Purified Mouse Anti-Human CD126 - Purified - Clone M5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mixture of U266, XG-1, and BWD41 cells

Klon: M5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IL-6 Receptor Antikörper (ABIN2688945)

Senaldi, Varnum, Sarmiento, Starnes, Lile, Scully, Guo, Elliott, McNinch, Shaklee, Freeman, Manu, Simonet, Boone, Chang: "Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11458-63, (1999) (PubMed).

Hirano, Nakajima, Hibi: "Signaling mechanisms through gp130: a model of the cytokine system." in: Cytokine & growth factor reviews, Vol. 8, Issue 4, pp. 241-52, (1998) (PubMed).

Keul, Heinrich, Müller-newen, Muller, Woo: "A possible role for soluble IL-6 receptor in the pathogenesis of systemic onset juvenile chronic arthritis." in: Cytokine, Vol. 10, Issue 9, pp. 729-34, (1998) (PubMed).

Heinrich, Behrmann, Müller-Newen, Schaper, Graeve: "Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway." in: The Biochemical journal, Vol. 334 ( Pt 2), pp. 297-314, (1998) (PubMed).

Salvi, Girasole, Pedrazzoni, Passeri, Giuliani, Minelli, Braverman, Roti: "Increased serum concentrations of interleukin-6 (IL-6) and soluble IL-6 receptor in patients with Graves' disease." in: The Journal of clinical endocrinology and metabolism, Vol. 81, Issue 8, pp. 2976-9, (1996) (PubMed).

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Liautard, Gaillard, Mani, Montero-Julian, Duperray, Lu, Jourdan, Klein, Brailly, Brochier: "Epitope analysis of human IL-6 receptor gp80 molecule with monoclonal antibodies." in: European cytokine network, Vol. 5, Issue 3, pp. 293-300, (1994) (PubMed).

Hibi, Murakami, Saito, Hirano, Taga, Kishimoto: "Molecular cloning and expression of an IL-6 signal transducer, gp130." in: Cell, Vol. 63, Issue 6, pp. 1149-57, (1991) (PubMed).

Van Snick: "Interleukin-6: an overview." in: Annual review of immunology, Vol. 8, pp. 253-78, (1990) (PubMed).

Taga, Hibi, Hirata, Yamasaki, Yasukawa, Matsuda, Hirano, Kishimoto: "Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130." in: Cell, Vol. 58, Issue 3, pp. 573-81, (1989) (PubMed).

Yamasaki, Taga, Hirata, Yawata, Kawanishi, Seed, Taniguchi, Hirano, Kishimoto: "Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor." in: Science (New York, N.Y.), Vol. 241, Issue 4867, pp. 825-8, (1988) (PubMed).

Taga, Kawanishi, Hardy, Hirano, Kishimoto: "Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression." in: The Journal of experimental medicine, Vol. 166, Issue 4, pp. 967-81, (1987) (PubMed).

Details zu IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Andere Bezeichnung: CD126 (IL6R Produkte)

Synonyme: IL6R antikoerper, il-6ra antikoerper, CD126 antikoerper, IL-6R-1 antikoerper, IL-6RA antikoerper, IL6Q antikoerper, IL6RA antikoerper, IL6RQ antikoerper, gp80 antikoerper, IL6R1 antikoerper, Il6ra antikoerper, IL-6Ralpha antikoerper, IL-6R antikoerper, Il6r antikoerper, interleukin 6 receptor antikoerper, interleukin 6 receptor, alpha antikoerper, IL6R antikoerper, il6r antikoerper, Il6r antikoerper, Il6ra antikoerper

Hintergrund: Synonyms: IL-6 Receptor α-chain

Pathways: JAK-STAT Signalweg, Autophagie, Growth Factor Binding, Cancer Immune Checkpoints