IL4 Receptor Antikörper

Produktnummer ABIN2688941

Produktdetails anti-IL4 Receptor Antikörper

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL4 Receptor Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut)

Marke: BD Pharmingen™

Produktmerkmale: The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c (left panel) and C57BL/6 (right panel) mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block (Cat. No. 553142, Rat IgG2b anti-mouse CD16/CD32) to block mouse Fc receptors. The cells were then stained with mIL4R-M1 (1 μg) followed by biotinylated RG7/1.30 (mouse anti-rat IgG2α, 0.25 μg, Cat. No. 553894) and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody (filled histograms) is compared to staining with the secondary reagents alone (open histograms).

BD Pharmingen™ Purified Rat Anti-Mouse CD124 - Purified - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: MIL4R

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IL4 Receptor Antikörper (ABIN2688941)

Gessner, Röllinghoff: "Biologic functions and signaling of the interleukin-4 receptor complexes." in: Immunobiology, Vol. 201, Issue 3-4, pp. 285-307, (2000) (PubMed).

Kubo, Yamashita, Abe, Tada, Okumura, Ransom, Nakayama: "CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2432-42, (1999) (PubMed).

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).

Beckmann, Schooley, Gallis, Vanden Bos, Friend, Alpert, Raunio, Prickett, Baker, Park: "Monoclonal antibodies block murine IL-4 receptor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4212-7, (1990) (PubMed).

Mosley, Beckmann, March, Idzerda, Gimpel, VandenBos, Friend, Alpert, Anderson, Jackson: "The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms." in: Cell, Vol. 59, Issue 2, pp. 335-48, (1989) (PubMed).

Lowenthal, Castle, Christiansen, Schreurs, Rennick, Arai, Hoy, Takebe, Howard: "Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 2, pp. 456-64, (1988) (PubMed).

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: CD124 (IL4R Produkte)

Synonyme: IL4R antikoerper, il-4ra antikoerper, zgc:113063 antikoerper, CD124 antikoerper, IL-4RA antikoerper, IL4RA antikoerper, Il4ra antikoerper, IL4 antikoerper, Il4r antikoerper, interleukin 4 receptor antikoerper, interleukin 4 receptor, tandem duplicate 1 antikoerper, interleukin 4 receptor, alpha antikoerper, IL4R antikoerper, il4r.1 antikoerper, Il4r antikoerper, Il4ra antikoerper

Hintergrund: Synonyms: IL-4 Receptor α chain

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process