IL4 Receptor Antikörper (Biotin)

Produktnummer ABIN2688938

Produktdetails anti-IL4 Receptor Antikörper (Biotin)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL4 Receptor Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut)

Marke: BD Pharmingen™

Produktmerkmale: The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block™ (Cat. No. 553142) to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 (0.125 μg/10^6 cells) followed by streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) and FITC anti-mouse B220 (0.06 μg, Cat. No. 553088) and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells (with the light-scattering characteristics of viable lymphocytes) are shown in the two-color dot plot (right panel). Staining with the biotinylated mIL4R-M1 antibody (right panel) is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody (0.125 μg, Cat. No. 553928) that is shown in the left panel.

BD Pharmingen™ Biotin Rat Anti-Mouse CD124 - Biotin - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CTLL-19.4 T Cell Line

Klon: MIL4R

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-IL4 Receptor Antikörper (Biotin) (ABIN2688938)

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, (1994) (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: CD124 (IL4R Produkte)

Synonyme: IL4R antikoerper, il-4ra antikoerper, zgc:113063 antikoerper, CD124 antikoerper, IL-4RA antikoerper, IL4RA antikoerper, Il4ra antikoerper, IL4 antikoerper, Il4r antikoerper, interleukin 4 receptor antikoerper, interleukin 4 receptor, tandem duplicate 1 antikoerper, interleukin 4 receptor, alpha antikoerper, IL4R antikoerper, il4r.1 antikoerper, Il4r antikoerper, Il4ra antikoerper

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process