IFNGR1 Antikörper

Produktnummer ABIN2688896

Produktdetails anti-IFNGR1 Antikörper

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IFNGR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Marke: BD Pharmingen™

Produktmerkmale: The GIR-94 antibody recognizes the extracellular region of the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γ receptor (IFN-γRα, aka, CD119). The functionally active-form of the human IFN-γ receptor consists of two (or more) subunits, with IFN-γRα responsible for IFN-γ binding and both the IFN-γR α and β chains required for the transduction of biologic responses. The IFN-γ receptor α chain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. The ability of this antibody to bind to IFN-γ receptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γRα purified from human placenta. The GIR-94 is a non-neutralizing antibody. Human PBMC (Left panel) and red blood cells (Right panel) were isolated by Lymphoprep (Nycomed) density centrifugation. The cells were stained with GIR-94 (1 μg, Cat. No. 558935) followed by biotinylated goat anti- mouse IgG (0.25 μg, Cat. No. 553999) and streptavidin -PE (0.015 μg, Cat No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the GIR-94 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in left panel are gated on the lymphocyte population defined by its light scattering characteristics.

BD Pharmingen™ Purified Mouse Anti-Human CD119 - Purified - Clone GIR-94 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IFN-gammaRalpha

Klon: GIR

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-IFNGR1 Antikörper (ABIN2688896)

Bach, Aguet, Schreiber: "The IFN gamma receptor: a paradigm for cytokine receptor signaling." in: Annual review of immunology, Vol. 15, pp. 563-91, (1997) (PubMed).

Greenlund, Schreiber, Goeddel, Pennica: "Interferon-gamma induces receptor dimerization in solution and on cells." in: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18103-10, (1993) (PubMed).

Sheehan, Calderon, Schreiber: "Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4231-7, (1988) (PubMed).

Details zu IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Andere Bezeichnung: CD119 (IFNGR1 Produkte)

Synonyme: CD119 antikoerper, IFNGR antikoerper, IFN-gammaR antikoerper, Ifgr antikoerper, Ifngr antikoerper, Nktar antikoerper, IFNGR1 antikoerper, MGC129098 antikoerper, ifngr1 antikoerper, MGC194858 antikoerper, MGC194873 antikoerper, zgc:194858 antikoerper, cd119 antikoerper, ifngr antikoerper, DKFZp469K1232 antikoerper, interferon gamma receptor 1 antikoerper, cytokine receptor family member B17 antikoerper, IFNGR1 antikoerper, Ifngr1 antikoerper, crfb17 antikoerper, ifngr1 antikoerper

Hintergrund: Synonyms: IFN-γ Receptor α chain

Pathways: Interferon-gamma Pathway