POLR2A Antikörper (Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa) (C-Term)

Details for Product anti-POLR2A Antibody No. ABIN268311, Anbieter: Anmelden zum Anzeigen
Antigen
  • NCU02103.1
  • 19.m02399
  • POLR2A
  • 220kDa
  • Rpb1
  • Rpo2-1
  • POLR2
  • POLRA
  • RPB1
  • RPBh1
  • RPO2
  • RPOL2
  • RpIILS
  • hRPB220
  • hsRPB1
  • DNA-directed RNA polymerase II largest subunit
  • DNA-directed RNA polymerase II subunit RPB1
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • polymerase (RNA) II (DNA directed) polypeptide A
  • NCU02103
  • BBOV_I003360
  • EDI_169920
  • CMU_000800
  • SJAG_01713
  • MGYG_01527
  • POLR2A
  • TP03_0185
  • EBI_22514
  • CGB_E5020W
  • polr2a
  • Polr2a
Epitop
C-Term, Large Subunit
22
17
13
8
8
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Yeast
153
76
72
36
28
28
20
15
15
13
8
8
5
4
4
3
2
2
1
1
1
Wirt
Maus
90
62
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser POLR2A Antikörper ist unkonjugiert
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
3
Applikation
Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
138
76
49
46
36
21
14
12
10
4
3
3
3
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
Klon 4H8
Isotyp IgG1
Spezifität This antibody recognizes the C-terminal repeat of the largest subunit of RNA polymerase II. It recognizes both unphosphorylated and phosphorylated forms.
Keine Kreuzreaktivität Primate
Reinigung Protein G purified
Andere Bezeichnung POLR2A (POLR2A Antibody Abstract)
Hintergrund Gene Symbol: POLR2A
Molekulargewicht Theoretical MW: 217 kDa
Gen-ID 5430
UniProt P24928
Pathways Regulatorische RNA Pathways
Applikationshinweise Western Blot 1-2 μg/mL, Chromatin Immunoprecipitation 1:10-1:500, Flow Cytometry 1 μg/mL, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:10-1:500, CyTOF-readyThis RNA polymerase II Antibody (4H8) is useful for Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation and Western blot. In WB a band can be seen at approximately 217 kDa. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Western Blot Protocol Specific for NB200-598
1. Perform SDS-PAGE on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: No Preservative
Konservierungsmittel Without preservative
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunocytochemistry (ICC) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody (ABIN268311) anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody
Immunofluorescence (IF) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody (ABIN268311) Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) [ABIN...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody (ABIN268311) Western Blot: RNA polymerase II Antibody (4H8) [ABIN268311] - Whole cell protein from...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody (ABIN268311) Western Blot: RNA polymerase II Antibody (4H8) [ABIN268311] - Western blot analysis o...
 image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa (POLR2A) (C-Term), (Large Subunit) antibody (ABIN268311) Flow (Intracellular): RNA Polymerase II/POLR2A Antibody (4H8) [ABIN268311] - An intra...
Produkt verwendet in: Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Kato, Okazaki, Iida, Nakayama, Murakami, Urano: "Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3." in: Scientific reports, Vol. 3, pp. 2186, 2013 (PubMed). (Probematerial (Species): Yeast). Weitere Details: Chromatin Immunoprecipitation

Chan, Teng, Corbett, Choudhury, Bassing, Schatz, Krangel: "Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 48, pp. E4628-37, 2013 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Cheng, Torkamani, Peng, Jones, Lerner: "Plasma membrane associated transcription of cytoplasmic DNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. 10827-31, 2012 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Walsh, Shupnik: "Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 2, pp. 237-50, 2009 (PubMed). (Probematerial (Species): Human). Weitere Details: Chromatin Immunoprecipitation

Reid, Svejstrup: "DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 29875-8, 2004 (PubMed). (Probematerial (Species): Yeast). Weitere Details: Western Blotting,Immunoprecipitation

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