Tumor Protein P53 (TP53) Antikörper

Antigen: Tumor Protein P53 (TP53)

Synonyme: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Klonalität: Monoklonal (X77)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN268282

Produktbeschreibung

Weitere Bezeichnung: P53 (TP53)

Gen-ID: 7157, 22059

Immunogen: Full length Xenopus p53 protein.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: X77

Beschreibung: P53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induceapoptosis in response to stress or DNA damage. Disruption of the p53 signallingpathway through various mechanisms is the most common alteration in human canceroccuring in over half of all tumors. The p53 protein is short lived and expressed at lowlevels in normal cells but accumulates and/or is activated in cells that have undergonegenotoxic damage or oncogene activation. Many tumor derived and transformed celllines express elevated levels of mutant p53 protein. Other genes also implicated in thedownstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax,Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. Alternate Names: anti-Cys 51 Stop antibody, anti-HGNC11998 antibody, anti-p53 Cellular Tumor Antigenantibody, anti-p53 Tumor Suppressor antibody, Phosphoproteinp53 antibody, anti-TP53antibody, anti-Transformation related protein 53 antibody, anti-TRP53 antibody,anti-Tumour Protein p53 antibody.

Spezifität: Detects a band at approximately 53kDa in HCT116 cell lysates. Species Reactivity: Cross-reacts with Human, Mouse and Xenopus laevis. Not yet tested in other species. Localization: Nuclear. Both nuclear and cytoplasmic in some cells.

Anwendungen

Applikationshinweise: WB: 1-10 ug/ml

Konzentration: 1 mg/ml

Buffer: PBS. Preservative: 0.08% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Transkriptionsfaktoren, Phospho-Antikörper, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Hellin, Bentires-Alj, Verlaet et al.: "Roles of nuclear factor-kappaB, p53, and p21/WAF1 in daunomycin-induced cell cycle arrest and apoptosis." in: The Journal of pharmacology and experimental therapeutics, Vol. 295, Issue 3, pp. 870-8, 2000 (PubMed).

Hussain, Harris: "p53 biological network: at the crossroads of the cellular-stress response pathway and molecular carcinogenesis." in: Journal of Nippon Medical School = Nihon Ika Daigaku zasshi, Vol. 73, Issue 2, pp. 54-64, 2006 (PubMed).

Pascreau, Eckerdt, Lewellyn et al.: "Phosphorylation of p53 is regulated by TPX2-Aurora A in xenopus oocytes." in: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5497-505, 2009 (PubMed).