C1q Antikörper

Antigen: C1q

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Human

Applikation: Radioimmunoassay (RIA)

Produktnummer: ABIN268087

Produktbeschreibung

Gen-ID: 712

Immunogen: Human C1q prepared form human plasma.Greater than 95% purity by SDS-PAGE.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: C1q is a sub-component of the first complement component C1, which is formed by theinteraction of C1q with the tetramer C1s-C1r-C1r-C1s.C1q is a member of a family of soluble collagen-like proteins and is composed of threebasic subunits termed A, B and C (27,550, 25,200, 23,800 Da respectively) whichasociate in the proportion 6 A-B subunits (52, 750 Da) and 3 C-C subunits (47,600 Da),to form whole C1q (459,300 Da). C1q binds to IgG and IgM immune complexes, thisactivates C1r and C1s, which are also bound to C1q and so initiates the classicalpathway of complement activation. Alternate Names: anti-C1QA antibody, anti-Complement C1q subcomponent subunit A antibody,anti-complement component 1 q subcomponent A chain antibody, anti-Complementcomponent 1 q subcomponent alpha polypeptide antibody, anti-complement componentC1q A chain antibody.

Spezifität: This product gives a single arc when tested by IEP against fresh human plasma.Identityhas been confirmed by double diffusion (Ouchterlony) vs fresh human plasma and aknown anti-human C1q. Species Reactivity: Cross-reacts with Human.Not yet tested in other species. Localization: Cytoplasmic and Secreted.

Anwendungen

Applikationshinweise: RID and Rocket IEP: 0.1uL antiserum/cm2 in gel vs 20uL fresh plasma, NT ? 1/2DoubleDiffusion: 10uL antiserum vs 20uL fresh plasmaSecond Antibody: 100uL antiserum vs10uL fresh plasma The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer(such as TBE or Tris-barbital pH >8.2) is recommended. Results can be enhanced bywashing completed agarose gels in saline, drying and finally stainingwith a protein stainsuch as coomassie blue.Suitability for use in immunohistochemical procedures,enzyme-linked immunosorbent assays and Western blot has not been assessed butusein such assays should not necessarily be excluded.

Buffer: Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01%Benzamidine,1mM EDTA

Lagerung: Store at 4C. Do not freeze.

Forschungsgebiet: Neurologie, Angeborene Immunität, Komplementfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar