Aralkylamine N-Acetyltransferase (AANAT) (pSer206) Antikörper

Antigen: Aralkylamine N-Acetyltransferase (AANAT)

Synonyme: SNAT, AA-NAT

Epitope: pSer206

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN267150

Produktbeschreibung

Weitere Bezeichnung: Serotonin acetylase pSer206

Immunogen: Synthetic peptide: C(198)HTFLRRNS(p)GC(208).Immunizing phosphopeptidecorresponds to C-terminal amino acid residues 198-208 of rat AA-NAT. This sequenceis completely conserved between rat and mouse AA-NAT protein.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase,AA-NAT) is a ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis.Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock.The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending onspecies among vertebrates. Studies have shown that AA-NAT is regulated by cAMP atboth the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites atThr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role inprotein inactivation via proteasome-dependent degradation.

Spezifität: This antibody detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.Phosphorylated:Ser206. Species Reactivity: Cross-reacts with Rat. Expected to cross-react with Mouse due to sequence homology.

Anwendungen

Applikationshinweise: Western Blot: use at a concentration of 0.5 ug/ml. By Western blot, this antibodydetects an ~23 kDa protein representing phosphorylated AA-NAT from rat pineal glandwhole protein extract.

Buffer: PBS, 1 mg/ml BSA. Preservative: 0.05% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Enzyme, Phospho-Antikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of serotonin AA-NAT in transfected 293T cell lysate using NB120-344.