Aralkylamine N-Acetyltransferase (AANAT) (pThr29) Antikörper

Antigen: Aralkylamine N-Acetyltransferase (AANAT)

Synonyme: SNAT, AA-NAT

Epitope: pThr29

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN267149

Produktbeschreibung

Weitere Bezeichnung: Serotonin acetylase pThr29

Immunogen: Synthetic peptide: C(24)QRRHT(p)LPAS(33).Immunizing phosphopeptide correspondsto N-terminal amino acid residues 24-33 of mouse AA-NAT.This sequence is completelyconserved between mouse, rat, and human AA-NAT protein.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase,AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis.Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock.The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending onspecies among vertebrates. Studies have shown that AA-NAT is regulated by cAMP atboth the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites atThr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role inprotein inactivation via proteasome-dependent degradation.

Spezifität: ABIN267149 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.Phosphorylated:Thr29. Species Reactivity: Cross-reacts with Rat and Mouse.Expected to cross-react with Human due to sequencehomology.Not yet tested in other species.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 0.5 ug/ml

Buffer: PBS. Preservative: 1 mg/ml BSA and 0.05% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1 shows a Western blot of phospho AA-NAT in transfected 293T cell lysate using ABIN267149.