Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Aufreinigung
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
C3AR is a single chain protein of 482 aa, with seven membrane-spanning regions and a molecular weight of 54 Kd. C3AR is expressed by neutrophils, eosinophils, mast cells, monocytes, dendritic cells and there are reports of C3AR expression in non-immune cells such as hematopoietic and mesenchymal stem cells, epithelial and endothelial cells. C3AR is coupled to heterotrimeric G proteins and after C3a binding, the signal transduced, results in chemotaxis, granule enzyme release and superoxide anion production.