Involucrin Antikörper
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- Target Alle Involucrin (IVL) Antikörper anzeigen
- Involucrin (IVL)
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Involucrin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody detects endogenous levels of Involucrin protein. (region surrounding Pro568)
- Kreuzreaktivität (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Aufreinigung
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product IVL Primärantikörper
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- Applikationshinweise
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ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Involucrin (IVL)
- Andere Bezeichnung
- Involucrin / IVL (IVL Produkte)
- Synonyme
- INV antikoerper, NPH2 antikoerper, NPHP2 antikoerper, 1110019C06Rik antikoerper, IVL antikoerper, Nphp2 antikoerper, inv antikoerper, involucrin antikoerper, inversin antikoerper, IVL antikoerper, INVS antikoerper, Ivl antikoerper, Invs antikoerper
- Hintergrund
- Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. Involucrin first appears in the cell cytosol but ultimately becomes cross-linked to membrane proteins by transglutaminase. During keratinocyte terminal differentiation glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent ε-(γ-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors. Involucrin is specifically expressed in Chinese hamster ovarian cells (fibroblasts), PtK2 rat kangaroo kidney cells (simple epithelial) and rat epidermal keratinocytes (stratifying squamous epithelial).
- Molekulargewicht
- approx. 68 kDa
- Gen-ID
- 3713
- NCBI Accession
- NP_005538
- UniProt
- P07476
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