Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta) Antikörper

Antigen: Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN264851

Produktbeschreibung

Produktmerkmale: Synonyms: HSP90AB1, HSP90B, HSPC2, HSPCB, HSP84, HSP-90, HSP-84, Heat shock protein HSP90-beta, HSP90AA1, HSP90A, HSPC1, HSPCA, HSP86, HSP-86, Renal carcinoma antigenNY-REN-38, Heat shock protein HSP 90-alpha

Weitere Bezeichnung: Heat Shock Protein 90 (HSP90) alpha/beta

Immunogen: Full length protein Hsp90

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Beschreibung: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryoticcells. From a functional perspective, hsp90 participates in the folding, assembly,maturation, and stabilization of specific proteins as an integral component of a chaperonecomplex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the mosthighly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out anumber of housekeeping functions – including controlling the activity, turnover, andtrafficking of a variety of proteins. Most of the hsp90-regulated proteins that have beendiscovered to date are involved in cell signaling (5-6). The number of proteins now know tointeract with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, andc-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases ofthe hepatitis B virus and telomerase. 5. When bound to ATP, Hsp90 interacts withco-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming acomplex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexeswith it. In a number of cases, variations in hsp90 expression or hsp90 mutation has beenshown to degrade signaling function via the protein or to impair a specific function of theprotein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such asgeldanamycin and radicicol, inhibit hsp90 function (7).

Spezifität: Detects 90kD proteins corresponding to the molecular mass of hsp90αβ. Species: Human, Rat, Mouse.

Anwendungen

Applikationshinweise: Western blot (1): 1: 20000-40000 (ECL). ELISA. Immonoprecipitation. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Reinigung: Serum

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar