ITGAL Antikörper (PE)

Produktnummer ABIN264311

Produktdetails anti-ITGAL Antikörper (PE)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGAL Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: This antibody reacts with LFA-1 alpha. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.

Produktmerkmale: Synonyms: Integrin alpha-L, LFA1, LFA-1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocytefunction-associated molecule 1 alpha chain, CD11 antigen-like family member A

Klon: OFYS

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Characterization of LFA-1 immunodeficiency. Monitoring of subsets in peripheral blood. Graft-versus-host disease. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Andere Bezeichnung: CD11a / ITGAL (ITGAL Produkte)

Synonyme: CD11A antikoerper, LFA-1 antikoerper, LFA1A antikoerper, (p180) antikoerper, Cd11a antikoerper, LFA-1A antikoerper, Ly-15 antikoerper, Ly-21 antikoerper, CD11a antikoerper, ITGAL antikoerper, integrin subunit alpha L antikoerper, integrin alpha L antikoerper, ITGAL antikoerper, Itgal antikoerper

Hintergrund: CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

Gen-ID: 3683

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex