T Cell Receptor (TCR) Vb 22 Antikörper

Antigen: T Cell Receptor (TCR) Vb 22

Klonalität: Monoklonal (Immu546)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Funktionale Studien (Func)

Produktnummer: ABIN264107

Produktbeschreibung

Produktmerkmale: Synonyms: TCR V beta-22, TCR Vb22

Immunogen: Murine T-cell hybridoma transfected with human V beta-22 gene segment

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: Immu546

Spezifität: This antibody reacts to variable beta-22 of human T-cell receptor also called TCRBV22S1according to the nomenclature from Wei et al. This antibody recognizes at least the IGRb03sequence. Has been further characterized by cell sorting on PBL using this monoclonalantibody followed by analysis of sorted cells by molecular biology. Analysis of alpha chainmRNA by PCR with a panel of alpha specific oligonucleotides shows transcripts for most Valpha sequences. Analysis of beta chain mRNA by anchored PCR and sequencing, onlyshows transcripts for IgRbo3 sequence. There is another V beta-22 sequence differing onlyby one amino acid, MARVB23. Though similar this sequence has not been found by thetechnique described above until now. If the antibody either does not recognize the other Vbeta-22 sequence, or if this sequence is of very low occurrence remains to be determined. The specificity has been confirmed at the First Human TcR Monoclonal Antibody Workshopin San Francisco in 1995. Species: Human. Others not tested. Add. Information: Studies have shown that anti-V beta-22 may be useful in T-cell repertoire studies. Superantigenic stimulation of T cells.

Anwendungen

Buffer: PBS containing 1mg/ml BSAReconstitution: Restore with 0.5ml of distilled water

Lagerung: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) of sodium azide is recommended for storage of thereconstituted form for up to one month at 2 - 8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar