T Cell Receptor (TCR) Vb 13.1 Antikörper

Antigen: T Cell Receptor (TCR) Vb 13.1

Klonalität: Monoklonal (Immu222)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN264103

Produktbeschreibung

Produktmerkmale: Synonyms: TCR V beta-13.1, TCR Vb13.1

Immunogen: Murine T cell hybridoma transfected with human V beta-13. 1 gene segment

Kreuzreaktivität: Human

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: Immu222

Spezifität: This antibody reacts to the variable beta-13. 1 chain of the T cell receptor also calledTCRBV13S1 according to the nomenclature from Wei et al. V beta-13. 1 is a complexsubfamily of the TCR. This antibody recognizes the V beta-13. 1 member (HBP34 sequence). The sequence V beta-13. 3 (IGRb14) is not recognized. The antibody was characterized onhuman T-cell clones and by cell sorting followed by molecular analysis of the sorted cells. All of 11 sequence analyzed were V beta-13. 1 subfamily cannot be formally excluded. It wasbeen recently shown that Immu222 could recognize V beta-13. 4 (Clone 4. 1 sequence) and Vbeta-13. 6 (IGRb16 sequence) subsets. No crossreactivity was detected with any of 18 V betaspecific antibodies (representing - 70% of the alpha/beta repertoire) on a cell line sortedfrom PBL and 100% positive with Immu222. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: T-cell repertoire studies. Fluorescent microscopy or flow cytometry: 2g / 5 x 10e5 cells/tests or 100l whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Reinigung: Purified

Buffer: PBS containing 1mg/ml BSAReconstitution: Restore with 0.5ml of distilled water

Lagerung: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to 1 month at 2 - 8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar