T Cell Receptor (TCR) Vb 11 Antikörper

Antigen: T Cell Receptor (TCR) Vb 11

Klonalität: Monoklonal (C21)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN264102

Produktbeschreibung

Produktmerkmale: Synonyms: TCR V beta-11, TCR Vb11

Immunogen: Human T-Cell clone

Kreuzreaktivität: Human

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: C21

Beschreibung: T cell receptors (TCR) recognize foreign antigens which have been processed as smallpeptides and bound to major histocompatibility complex (MHC) molecules at the surfaceof antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alphaand one beta chain or one delta and one gamma chain. This region represents the germlineorganization of the T cell receptor beta locus. The beta locus includes V (variable), J(joining), diversity (D), and C (constant) segments. During T cell development, the betachain is synthesized by a recombination event at the DNA level joining a D segment with a Jsegment, a V segment is then joined to the D-J gene. The C segment is later joined bysplicing at the RNA level.

Spezifität: This antibody reacts to the variable beta-11 chain of the T cell receptor also calledTCRBV11S1 according to the nomenclature from Wei et al. Two V beta-11 sequences havebeen described, PL3. 12 and PH15. These sequences differ only in their leader sequence andtherefore lead to the same mature protein. C21 recognizes the gene product of thesesequences. It has been characterized on human T cell clones and by extensive cell sortingfollowed by molecular biology analysis. V beta-11 has been shown to be expanded in aclonal fashion in the CD3+ CD8- CD4- cell population in PBL of several donors. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: T-cell repertoire studies. Flow cytometry: 2g/5x10e5 cells/tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Reinigung: Purified

Buffer: PBS containing 1mg/ml BSAReconstitution: Restore with 0.5ml of distilled water

Lagerung: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to 1 month at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Immunologie, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar