Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta) Antikörper

Antigen: Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta)

Klonalität: Monoklonal (Hyb-K41220A)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN263958

Produktbeschreibung

Produktmerkmale: Synonyms: HSP90AB1, HSP90B, HSPC2, HSPCB, HSP84, HSP-90, HSP-84, Heat shock protein HSP90-beta, HSP90AA1, HSP90A, HSPC1, HSPCA, HSP86, HSP-86, Renal carcinoma antigenNY-REN-38, Heat shock protein HSP 90-alpha

Weitere Bezeichnung: Heat Shock Protein 90 (HSP90) alpha/beta

Gen-ID: 3320

Swiss-Prot: P07900

Immunogen: Recombinant Human hsp90 alpha

Kreuzreaktivität: Human, Ratte (Rattus), Hefe (Saccharomyces Cerevisiae), Schizosaccharomyces pombe (S. pombe)

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: Hyb-K41220A

Beschreibung: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understoodto exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite thesimilarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly asa monomer. (2) From a functional perspective, hsp90 participates in the folding, assembly,maturation, and stabilization of specific proteins as an integral component of a chaperonecomplex. (3-6) Furthermore, Hsp90 is highly conserved between species, having 60% and78% amino acid similarity between mammalian and the corresponding yeast andDrosophila proteins, respectively. Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryoticcells. Despite its label of being a heat-shock protein, hsp90 is one of the most highlyexpressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a numberof housekeeping functions – including controlling the activity, turnover, and trafficking of avariety of proteins. Most of the hsp90-regulated proteins that have been discovered todate are involved in cell signaling (7-8). The number of proteins now know to interact withHsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf,transcriptional regulators such as p53 and steroid receptors, and the polymerases of thehepatitis B virus and telomerase. 5 When bound to ATP, Hsp90 interacts withco-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming acomplex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexeswith it. In a number of cases, variations in hsp90 expression or hsp90 mutation has beenshown to degrade signaling function via the protein or to impair a specific function of theprotein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such asgeldanamycin and radicicol, inhibit hsp90 function (9).

Spezifität: Detects 90kD proteins corresponding to the molecular mass of HSP90 alpha or beta. Species: Human (beta-specific), Rat (Liver), S. cerevisiae, S. pombe. Limited or no crossreactivityto rice or P. caudatum (Ref. 10). Others not tested.

Anwendungen

Applikationshinweise: ELISA. (Ref.1)Western blot: 1 µg/ml was sufficient for detection of Hsp90alpha/beta by Western Blot in20 µg of HeLa lysate. (Ref.1, 10)Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.2 containing 50% Glycerol and 0.09% Sodium Azide aspreservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar