Schwein anti-Human IgE (Fc Region) Antikörper (FITC)
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- Target Alle IgE Produkte
- IgE
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Bindungsspezifität
- Fc Region
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Reaktivität
- Human
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Wirt
- Schwein
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Klonalität
- Polyklonal
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Konjugat
- FITC
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Applikation
- ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- Human IgE Fc specific.
- Aufreinigung
- Purified IgG
- Immunogen
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Pools of purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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- Applikationshinweise
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Approved: ELISA, ICC, IF, IHC, IHC-Fr
Usage: The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against human serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to identify and measure IgE in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2. No preservative added.
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IgE
- Abstract
- IgE Produkte
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