Ziege anti-Human IgA, IgG, IgM Antikörper (FITC)

Produktnummer ABIN2622482

Produktdetails

Target: IgA, IgG, IgM

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: FITC

Applikation: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Human IgG, IgA and IgM, heavy and light chains.

Aufreinigung: Purified IgG

Immunogen: Purified polyclonal human IgG, and IgA and IgM containing fractions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, ICC, IHC, IHC-Fr

Usage: The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of human origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgA + IgG + IgM (IgA, IgG, IgM Produkte)

Substanzklasse: Antibody

Hintergrund: This antibody detects: Human IgA + IgG + IgM