L-DOPA Antikörper

Antigen: L-DOPA

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Alle Spezies

Applikation: Enzyme Immunoassay (EIA)

Produktnummer: ABIN260083

Produktbeschreibung

Immunogen: Synthetic L-Dopa conjugated to BSA via glutaraldehyde.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: L Dopa is an intermediate in dopamine biosynthesis. Clinically, L Dopa is used in themanagement of Parkinson',s disease. It is used as a prodrug to increasedopamine levels since it is able to cross the blood-brain barrier whereas dopamine itselfcannot. Once L Dopa has entered the central nervous system (CNS), it is metabolisedto dopamine by aromatic L amino acid decarboxylase. This also occurs in the peripheraltissues, causing adverse effects and decreasing the available dopamine to the CNS, soit is standard practice to co administer a peripheral DOPA decarboxylase inhibitor andoften a catechol-O-methyl transferase (COMT) inhibitor. The antiserum was tested usingthe free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPAantibodies gave a good staining between 1/2000-1/5000 dilution in these areas. Alternate Names: anti-3 4 dihydroxy L phenylalanine antibody, anti-L dihydroxyphenylalanine antibody,anti-Levodopa antibody.

Spezifität: Specific for conjugated L-dihydroxyphenylalanine (L-DOPA). The following reactiviteswere determined by competition ELISA:CompoundCross reactivity ratioL-Dopa-G-BSA1alpha-methyl-L-Dopa-G-BSA1/2,2003-O-methyl-L-Dopa-G-BSA1/50,000Dopamine-G-BSA>1/50,000Noradrenaline-G-BSA>1/50,000Tyrosine-G-BSA>1/50,000L-Dopa-G-BSA concentration/other conjugated catecholamine concentration at halfdisplacement.L-Dopa is an amino acid that is converted to dopamine in the brain. It isoften given to patients in order to alleviate some of the symptoms of Parkinson',sdisease as, unlike dopamine itself, it can cross the blood-brain barrier. Species Reactivity: Reacts with Broad.

Anwendungen

Applikationshinweise: Suggested working dilutions: ELISA 1:5000 - 1:10000

Buffer: Phosphate buffered saline, pH 7.2. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar