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CD14 Molecule (CD14) Antikörper (APC)

Antigen

CD14 Molecule (CD14)

Synonyme CD14
Klonalität Monoklonal (MEM-18)
Wirt
Reaktivität
Alternativen

Human, Affe

Konjugat
Applikation
Alternativen Durchflusszytometrie (FACS)
Produktnummer ABIN259688
Menge 100 Tests
Preis 370,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung

Weitere Bezeichnung CD14
Gen-ID 929
Swiss-Prot 5q311
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from theurine of a patient suffering from proteinuria. Reference Sequence: 5q31.1.
Isotyp IgG1
Klon MEM-18
Beschreibung CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface ofmature monocytes, macrophages, and neutrophils, where serves as a multifunctionallipopolysaccharide receptor, it is also released to the serum both as a secreted andenzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in areaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serumprotein. The soluble sCD14 is able to discriminate slight structural differences betweenlipopolysaccharides and is important for neutralization of serum allochthonouslipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic,inflammatory and infectious processes.
Spezifität The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes,macrophages and weakly on granulocytes, also expressed by most tissuemacrophages. In human, the epitope recognized by MEM-18 is located between aminoacids 57-64.HLDA III, WS Code M 253HLDA IV, WS Code M 314HLDA V, WS Code M MA087HLDA VI, WS Code M MA95. Species Reactivity: Cross reacts with human and primate. Not tested on other species.

Anwendungen

Applikationshinweise The reagent is designed for Flow Cytometry analysis of human blood cells using 10 mlreagent / 100 ml of whole blood or 10^6 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodiumazide and 0.2% (w
Lagerung Store in the dark at 2-8C. Do not freeze. Avoid prolonged exposure to light. Do not useafter expiration date stamped on vial label. Short-term exposure to room temperatureshould not affect the quality of the reagent.However, if reagent is stored under
Forschungsgebiet Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "CD14 Molecule (CD14)", Typ "Antikörper" finden
Wirte Maus (365), Kaninchen (41), Ratte (35), Ziege (9), Huhn (3)
Reaktivitäten Human (388), Maus (63), Hund (54), Affe (40), Schwein (37), Rind (Kuh) (32), Schaf (28), Cynomolgus (25), Rhesusaffen (25), Kaninchen (24), Primaten (19), Ratte (Rattus) (19), Primate (14), Katze (9), Ziege (9), Pferd (2), Pavian (1), Huhn (1), Schimpanse (1), Llama (1)
Applikationen Durchflusszytometrie (FACS) (369), Western Blot (WB) (71), Immunpräzipitation (IP) (58), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (46), ELISA (37), Immunfluoreszenz (IF) (35), Enzyme Immunoassay (EIA) (32), Funktionale Studien (Func) (32), Immunhistochemie (IHC) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (27), Blockierende Antikörper (Inhibition) (5), ELISA (Detektionsantikörper) (5), Immunzytochemie (ICC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (55), PE (46), Biotin (39), APC (26), PerCP,Cy5.5 (10), Pacific Blue (8), RPE (8), Alexa Fluor 488 (6), PE,Cy7 (6), APC,Cy7 (5), Alexa Fluor 647 (5), Cy5.5 (5), Alexa Fluor 700 (4), Cy5 (2), Cy7,PE (2), EFluor 450 (2), EFluor 605NC (2), PE,Cy5 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), CF (1), Cy3 (1), Cy7 (1), DY590 (1), DY647 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitope C-Term (2), N-Term (2)