Tenascin C (TNC) (Domain B) Antikörper

Details zu Produkt Nr. ABIN259023, Anbieter: Anmelden zum Anzeigen
Antigen
  • tn
  • 150-225
  • GMEM
  • GP
  • HXB
  • JI
  • TN
  • TN-C
  • AI528729
  • C130033P17Rik
  • Hxb
  • Ten
  • cytotactin
  • tenascin-C
  • P230
  • tenc
  • wu:fk04d02
  • tenascin C
  • tnc
  • TNC
  • MCYG_00667
  • Tnc
Alternativen
anti-Ratte (Rattus) Tenascin C Antikörper für Cytometry by Time of Flight
Epitop
Domain B
18
8
7
6
5
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
267
81
70
5
3
2
1
Wirt
Maus
148
117
13
4
4
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
20
18
14
5
5
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Applikation
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
153
120
101
66
60
42
38
38
27
26
7
5
5
5
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Immunogen Recombinant human Tenascin C [Swiss-Prot# P24821].
Klon 4C8MS
Isotyp IgG1
Spezifität This antibody specifically reacts with Domain B on FNIII repeats of Tenascin C.
Produktmerkmale Tenascin C is an extracellular matrix protein that is expressed in various tissues during development, disease or injury. Tenascin C inhibits HIV infection in immune cells by binding to a chemokine coreceptor site on the HIV-1 envelope protein, thereby blocking the virus' entry into the host cells. Tenascin C expression in the stroma of certain tumors is often associated with a poor prognosis.
Reinigung Protein A purified
Andere Bezeichnung Tenascin C (TNC Antibody Abstract)
Hintergrund Gene Symbol: TNC
Gen-ID 3371
UniProt P24821
Forschungsgebiet Stem Cells, Extracellular Matrix
Pathways Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
Applikationshinweise Western Blot 5 μg/mL, Flow Cytometry 1 μg per million cells, ELISA, Immunohistochemistry 1:25 - 1:50, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25 - 1:50, Immunohistochemistry-Frozen, CyTOF-readySee PMID: 21871891 for the ICC/IF use of thsi product. For its use in IHC-P, it is recommended to incubate primary antibody for atleast 2 hours at room temperature followed by ON incubation at 4C.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Immunohistochemistry-Paraffin Embedded Sections Protocol Specific for NB110-68136: Tenascin C Antibody (4C8MS)Immunohistochemistry-Paraffin Embedded Sections for NB110-68136Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Tenascin C (TNC) (Domain B) Antikörper (ABIN259023) anti-Tenascin C (TNC) (Domain B) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tenascin C (TNC) (Domain B) Antikörper (ABIN259023) Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [ABIN2590236] - IHC analys...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tenascin C (TNC) (Domain B) Antikörper (ABIN259023) Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [ABIN2590236] - IHC analys...
Flow Cytometry (FACS) image for anti-Tenascin C (TNC) (Domain B) Antikörper (ABIN259023) Flow Cytometry: Tenascin C Antibody (4C8MS) [ABIN2590236] - Intracellular flow cytome...
Produkt verwendet in: Bhattacharyya, Wang, Morales-Nebreda, Feng, Wu, Zhou, Lafyatis, Lee, Hinchcliff, Feghali-Bostwick, Lakota, Budinger, Raparia, Tamaki, Varga: "Tenascin-C drives persistence of organ fibrosis." in: Nature communications, Vol. 7, pp. 11703, 2016 (PubMed). Weitere Details: Western Blotting,Immunoprecipitation

Janune, Abd El Kader, Aoyama, Nishida, Tabata, Kubota, Takigawa: "Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage." in: Journal of bone and mineral metabolism, 2016 (PubMed).

de Sousa, Gurgel, Ramos, Trindade, de Faro Valverde, Carneiro, Cangussú, Pinheiro, Dos Santos: "Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats--a preliminary study." in: Acta histochemica, Vol. 116, Issue 7, pp. 1185-91, 2014 (PubMed). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Ago, Kemmochi, Morita, Yafune, Shiraki, Mitsumori, Shibutani: "Direct progression of capsular invasive carcinomas from subcapsular focal hyperplasias induced by hypothyroidism-mediated tumor promotion in a rat two-stage thyroid carcinogenesis model." in: Journal of cancer research and clinical oncology, Vol. 139, Issue 3, pp. 395-401, 2013 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry

James, Ross, Nicola, Steele, Ambalavanan: "VARA attenuates hyperoxia-induced impaired alveolar development and lung function in newborn mice." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 304, Issue 11, pp. L803-12, 2013 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Kemmochi, Shimamoto, Shiraki, Onda, Hasumi, Suzuki, Mitsumori, Shibutani: "Promoting effects of carminic acid-enriched cochineal extracts on capsular invasive thyroid carcinomas through targeting activation of angiogenesis in rats." in: The Journal of toxicological sciences, Vol. 37, Issue 3, pp. 475-82, 2012 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry

Kemmochi, Yamamichi, Shimamoto, Onda, Hasumi, Suzuki, Mitsumori, Shibutani: "Lac color inhibits development of rat thyroid carcinomas through targeting activation of plasma hyaluronan-binding protein." in: Experimental biology and medicine (Maywood, N.J.), Vol. 237, Issue 6, pp. 728-38, 2012 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry

Yoshimura, Michishita, Ohkusu-Tsukada, Takahashi: "Increased presence of stromal myofibroblasts and tenascin-C with malignant progression in canine mammary tumors." in: Veterinary pathology, Vol. 48, Issue 1, pp. 313-21, 2011 (PubMed).

Balasenthil, Chen, Lott, Chen, Carter, Grizzle, Frazier, Sen, Killary: "A migration signature and plasma biomarker panel for pancreatic adenocarcinoma." in: Cancer prevention research (Philadelphia, Pa.), Vol. 4, Issue 1, pp. 137-49, 2011 (PubMed). (Probematerial (Species): Human). Weitere Details: ELISA

Yamamoto, Okui, Tatebe, Shinohara, Hirata: "Regeneration of the perineurium after microsurgical resection examined with immunolabeling for tenascin-C and alpha smooth muscle actin." in: Journal of anatomy, Vol. 218, Issue 4, pp. 413-25, 2011 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Janune, Kubota, Nishida, Kawaki, Perbal, Iida, Takigawa: "Novel effects of CCN3 that may direct the differentiation of chondrocytes." in: FEBS letters, Vol. 585, Issue 19, pp. 3033-40, 2011 (PubMed).

Guttery, Shaw, Lloyd, Pringle, Walker: "Expression of tenascin-C and its isoforms in the breast." in: Cancer metastasis reviews, Vol. 29, Issue 4, pp. 595-606, 2010 (PubMed).

Richter, Tost, Franz, Altendorf-Hofmann, Junker, Borsi, Neri, Kosmehl, Wunderlich, Berndt: "B and C domain containing tenascin-C: urinary markers for invasiveness of urothelial carcinoma of the urinary bladder?" in: Journal of cancer research and clinical oncology, Vol. 135, Issue 10, pp. 1351-8, 2009 (PubMed). (Probematerial (Species): Human). Weitere Details: ELISA,Immunohistochemistry (Frozen Sections),Immunohistochemistry

El-Karef, Kaito, Tanaka, Ikeda, Nishioka, Fujita, Inada, Adachi, Kawada, Nakajima, Imanaka-Yoshida, Yoshida: "Expression of large tenascin-C splice variants by hepatic stellate cells/myofibroblasts in chronic hepatitis C." in: Journal of hepatology, Vol. 46, Issue 4, pp. 664-73, 2007 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunohistochemistry

Kääriäinen, Nummela, Soikkeli, Yin, Lukk, Jahkola, Virolainen, Ora, Ukkonen, Saksela, Hölttä: "Switch to an invasive growth phase in melanoma is associated with tenascin-C, fibronectin, and procollagen-I forming specific channel structures for invasion." in: The Journal of pathology, Vol. 210, Issue 2, pp. 181-91, 2006 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Tamaoki, Imanaka-Yoshida, Yokoyama, Nishioka, Inada, Hiroe, Sakakura, Yoshida: "Tenascin-C regulates recruitment of myofibroblasts during tissue repair after myocardial injury." in: The American journal of pathology, Vol. 167, Issue 1, pp. 71-80, 2005 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Tsunoda, Inada, Kalembeyi, Imanaka-Yoshida, Sakakibara, Okada, Katsuta, Sakakura, Majima, Yoshida: "Involvement of large tenascin-C splice variants in breast cancer progression." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1857-67, 2003 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)