SLC9A3 Antikörper (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3) (C-Term)

Details for Product anti-SLC9A3 Antibody No. ABIN258888, Anbieter: Anmelden zum Anzeigen
Antigen
  • SLC9A3
  • 9030624O13Rik
  • AI930210
  • NHE-3
  • NHE3
  • Nhe3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • sodium/hydrogen exchanger 3 (nhe3)
  • Sodium/hydrogen exchanger 3
  • Na/H exchanger 3
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3
  • SLC9A3
  • AaeL_AAEL001503
  • GL50803_102647
  • Smp_045680
  • NHE-3
  • Slc9a3
Alternativen
anti-Ratte (Rattus) SLC9A3 Antikörper für ELISA
Epitop
C-Term
31
20
19
2
2
2
2
1
1
1
1
Reaktivität
Kaninchen, Ratte (Rattus)
136
84
62
39
38
36
4
1
1
1
1
Wirt
Maus
79
72
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser SLC9A3 Antikörper ist unkonjugiert
10
8
7
6
6
6
5
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
144
111
79
51
37
25
21
16
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Immunogen Fusion protein representing the C-terminal 131 amino acids of rabbit NHE3. [UniProt# P26432]
Klon 19F5
Isotyp IgG1
Spezifität This antibody is specific for NHE3. It does not show any cross-reactivity with any other NHE isoforms.
Keine Kreuzreaktivität Human
Reinigung Protein G purified
Andere Bezeichnung NHE3/SLC9A3 (SLC9A3 Antibody Abstract)
Hintergrund Gene Symbol: SLC9A3
Molekulargewicht Theoretical MW: 93 kDa
Gen-ID 6550
UniProt P26432
Pathways Proton Transport
Applikationshinweise Western Blot 0.5 μg/mL, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500This NHE3 antibody is useful for Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections and Western blot, where a band can be seen at ~93 kDa. Immunocytochemistry/Immunofluorescence and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Sodium / Hydrogen Exchanger 3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the mouse anti-NHE3 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-SLC9A3 Antikörper (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3) (C-Term) (ABIN258888) Detection of NHE3 in rat kidney lysate using ABIN258888.
Produkt verwendet in: Saad, Zhang, Yong, Yaghobian, Wong, Kelly, Chen, Pollock: "Role of the EGF receptor in PPARγ-mediated sodium and water transport in human proximal tubule cells." in: Diabetologia, Vol. 56, Issue 5, pp. 1174-82, 2013 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Kocinsky, Girardi, Biemesderfer, Nguyen, Mentone, Orlowski, Aronson: "Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites." in: American journal of physiology. Renal physiology, Vol. 289, Issue 2, pp. F249-58, 2005 (PubMed).

Sabolić, Herak-Kramberger, Ljubojević, Biemesderfer, Brown: "NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats." in: Kidney international, Vol. 61, Issue 4, pp. 1351-64, 2002 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Bagnis, Marsolais, Biemesderfer, Laprade, Breton: "Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis." in: American journal of physiology. Renal physiology, Vol. 280, Issue 3, pp. F426-36, 2001 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry

Girardi, Titan, Malnic, Rebouças: "Chronic effect of parathyroid hormone on NHE3 expression in rat renal proximal tubules." in: Kidney international, Vol. 58, Issue 4, pp. 1623-31, 2000 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting,Immunohistochemistry

Biemesderfer, Nagy, DeGray, Aronson: "Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17518-24, 1999 (PubMed). (Probematerial (Species): Rabbit). Weitere Details: Immunoprecipitation

Zhang, Norian, Magyar, Holstein-Rathlou, Mircheff, McDonough: "In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition." in: The American journal of physiology, Vol. 276, Issue 5 Pt 2, pp. F711-9, 1999 (PubMed). (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Biemesderfer, DeGray, Aronson: "Membrane topology of NHE3. Epitopes within the carboxyl-terminal hydrophilic domain are exoplasmic." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12391-6, 1998 (PubMed). (Probematerial (Species): Rabbit). Weitere Details: Western Blotting,Immunoprecipitation,ELISA

Biemesderfer, Rutherford, Nagy, Pizzonia, Abu-Alfa, Aronson: "Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney." in: The American journal of physiology, Vol. 273, Issue 2 Pt 2, pp. F289-99, 1997 (PubMed). Weitere Details: Immunocytochemistry,Western Blotting,ELISA,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Haben Sie etwas anderes gesucht?