Luciferase (Photinus Pyralis) Antikörper

Antigen: Luciferase (Photinus Pyralis)

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunfluoreszenz (IF)

Produktnummer: ABIN257965

Produktbeschreibung

Immunogen: Full length native protein (purified) (Firefly (Photinus pyralis)).

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Analysis of gene expression is most commonly assayed by transient transfection. Thesesystems are usually based on the use of fusion genes which are inserted into cells, andexpression of the gene is assayed within 48 hours after introduction of DNA. Usually thefusion consists of the promoter binding site or enhancer sequence under study which isattached to a reporter gene. The amount of the reporter protein synthesized under theexperimental conditions, is presumed to reflect the ability of the sequences studied todirect or promote transcription. Several enzymes are commonly used as reporterproteins, among them are chloramphenicol acetyl transferase (CAT),â,-galactosidase, human growth hormone (hGH), and luciferase. Luciferasehas become one of the more widely used reporter enzymes. The reporter plasmidcontains the gene from the firefly Photinus pyralis. The enzyme catalyzes abioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP.Mixing these reagents with the cell extract containing luciferase, results in a flash of lightthat decays rapidly. This light can be detected by a luminometer. The total light emissionis proportional to the luciferase activity of the sample. The luciferase assay is fast andsensitive and does not require a radioactive substrate as is in the CAT assay. Adisadvantage of the luciferase assay is that it requires a rather expensive instrument,the luminometer, to measure enzyme activity. In addition, this assay lacks reproducibilitybetween samples, largely due to the rapid kinetics of the emission. Alternate Names: Anti-ec 1.13.12.7 antibody, Anti-Luciferase antibody, Anti-Luciferin 4 monooxygenaseantibody Anti-firefly luciferase antibody, Anti-luciferase antibody, Anti-firefly antibody.

Spezifität: The use of an antibody to detect luciferase can provide an alternative detection assay.This assay directly detects luciferase protein levels, and thus has the advantage that itdoes not require luciferase activity and is not dependent on rapid kinetics. Moreover,antibodies can detect the luciferase enzyme expression in situ, providing a means tostudy the localized signal sequences using luciferase as a reporter gene. Species Reactivity: Cross-reacts with Firefly (Photinus pyralis) Luciferase. Localization: Peroxisomal.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunofluorescence 10 ug/ml

Buffer: Constituents: 0.01M PBS. pH 7.4. Preservative: 15mM Sodium Azide

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: Transfektion, Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar