Agrin (AGRN) Antikörper
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| Synonyme |
Agrin, AGR, FLJ45064, AGRN, AGRIN, agrn, wu:fb57e07 |
| Klonalität |
Monoklonal (AGR 131) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Radioimmunoassay (RIA), Western Blot (WB)
Immunfluoreszenz (IF) (22), Durchflusszytometrie (FACS) (18), Western Blot (WB) (7), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), ELISA (3), Immunpräzipitation (IP) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Paraffinschnitte) (IHC (p)) (2), Enzyme Immunoassay (EIA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN257890 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
400,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Weitere Bezeichnung
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Agrin
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Immunogen
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Recombinant rat agrin, C-terminal construct.
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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AGR 131
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Beschreibung
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Agrin is an essential extracellular matrix component which promotes clustering ofnicotinic acetylcholine receptors (nAChRs) and other proteins during development at theneuromuscular junction. Agrin, MuSK and Rapsyn are all essential components forAChR aggregation, through an unknown mechanism. The C-terminal region of agrin isreleased into the medium, interacts with receptors on the muscle surface and inducesAChR aggregation. The central region contains two O-linked glycosylation sites and adomain homologous to domain III of laminin. The N-terminal region anchors agrin to theextracellular matrix via other basal membrane components. This region also contains aprotease inhibitor domain and glycosaminoglycan attachment sites, increasing thepredicted MW from 200kDa to ~600kDa. Alternate Names: anti-AGRN antibody, anti-FLJ45064 antibody, anti-OTTHUMP00000044043 antibody.
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Spezifität
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Ab12362 detects all isoforms of agrin. This antibody recognizes all N1 isoforms but,consistent with the epitope mapping investigations, fails to see the N4 protein.Furthermore, the antibody will detect the X, Y and Z splice variants. Species Reactivity: Cross-reacts with Mouse and Rat. Not yet tested in other species. Localization: Synaptic basal lamina at the neuromuscular junction.
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Applikationshinweise
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Suggested working dilutions: Western Blot 2 ug/ml,Immunocytochemistry 1/50 - 1/1000,RID
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Konzentration
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1 mg/ml
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Buffer
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50% Glycerol, PBS, 0.1mM PMSF. pH 7.2. Preservative: No Preservative.
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Lagerung
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
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Forschungsgebiet
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Neurologie
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Agrin (AGRN)", Typ "Antikörper" finden
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Wirte
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Kaninchen (20), Maus (9), Schaf (1)
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Reaktivitäten
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Ratte (Rattus) (28), Maus (26), Human (20)
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Applikationen
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Immunfluoreszenz (IF) (22), Durchflusszytometrie (FACS) (18), Western Blot (WB) (7), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), ELISA (3), Immunpräzipitation (IP) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Paraffinschnitte) (IHC (p)) (2), Enzyme Immunoassay (EIA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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