Activating Transcription Factor 2 (ATF2) Antikörper

Antigen: Activating Transcription Factor 2 (ATF2)

Synonyme: HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human, Ratte (Rattus)

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN257655

Produktbeschreibung

Weitere Bezeichnung: ATF2

Gen-ID: 1386, 81647

Swiss-Prot: NP_001871

Immunogen: Synthetic peptide with sequence IVMAPSSQSQPSGS representing the C-terminus ofthe human protein (residues 492-505), according to NP_001871. Reference Sequence: NP_001871.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a memberof the ATF/CREB family of basic region leucine zipper DNA binding proteins thatregulates transcription by binding to a consensus cAMP response element (CRE) in thepromoter of various viral and cellular genes. Many of these genes are important in cellgrowth and differentiation, and in stress and immune responses. ATF2 is a nuclearprotein that binds DNA as a dimer and can form dimers with members of the ATF/CREBand Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as ahomodimer. Several isoforms of ATF2 arise by differential splicing. The stable native fulllength ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecularinteraction of its carboxy terminal DNA binding domain with the amino terminaltransactivation domain. Following dimerization ATF2 becomes a short lived protein thatundergoes ubiquitination and proteolysis, seemingly in a proteinphosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2occurs following cellular stress induced by several genotoxic agents, inflammatorycytokines, and UV irradiation. This activation requires phosphorylation of two threonineresidues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantlyexpressed in brain. Alternate Names: anti-Activating Transcription Factor 2 antibody, anti-cAMP Response Element BindingProtein 2 antibody, anti-CRE BP1 antibody, anti-CREB 2 antibody, anti-CREB2antibody, anti-CREBP1 antibody, anti-HB 16 antibody, anti-HB16 antibody,anti-MGC111558 antibody, anti-mXBP antibody, anti-TREB 7 antibody, anti-TREB7antibody.

Spezifität: Species Reactivity: Cross-reacts with Human, Rat. Localization: Nuclear.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 0.5-2ug/ml

Konzentration: 0.5 mg/ml

Buffer: Tris saline containing [pH7.3] with 0.5% BSA. Preservative: 0.02% Na azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Immunologie, Angeborene Immunität, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot: ATF2 Antibody [ABIN257655] - ABIN257655 Staining (0.5ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Online Catalogue 14-3-3 sigma to CYBRD1 Goat Anti-ATF2 Antibody

ABIN257655 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.