Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antikörper

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Klonalität: Monoklonal (EPR1977Y)

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN256816

Produktbeschreibung

Weitere Bezeichnung: GAPDH

Gen-ID: 2597, 24383

Swiss-Prot: P04406

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EPR1977Y

Beschreibung: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was considered a classicalglycolytic protein involved exclusively in cytosolic energy production. However, recentevidence suggests that it is a multifunctional protein displaying diverse activities distinctfrom its conventional metabolic role. These new roles for GAPDH may be dependent onits subcellular localization, oligomeric state or on the proliferative state of the cell.Results suggest that human cells contain significant intracellular levels of enzymaticallyinactive GAPDH which is age-independent. The possibility is considered that thefunctional diversity of GAPDH may be mediated either by posttranslational alteration orby subcellular protein:protein and/or protein:nucleic acid interactions (1). GAPDH mRNAlevels, protein, and enzymatic activity have been shown to increase increase in 3T3cells after exposure to physiological concentrations of insulin (2).

Spezifität: A synthetic peptide corresponding to residues in human GAPDH was used as animmunogen. Species Reactivity: This antibody is reactive against Human, Mouse, Rat.

Synonyme: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:1000-200, Immunohistochemistry 1:100-250, Immunocytochemistry 1:50-100, Immunoprecipitation 1:40

Buffer: Preservative: 0.01% Sodium Azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Krebs, Metabolismus, Alzheimer-Krankheit (Morbus Alzheimer), Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

A. Western blot analysis on (A) NIH/3T3 or (B) HeLa cell lysate using anti-GAPDH RabMAb, dilution 1:2000.

B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-GAPDH RabMAb.

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (Image 3)