Cytokeratin 5 (KRT5) Antikörper

Antigen: Cytokeratin 5 (KRT5)

Synonyme: KRT79, CK5, KRT5, K5, Tfip8, Krt2-5, AW146334, 3300001P10Rik, DDD, EBS2, KRT5A

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN256786

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 5

Gen-ID: 3852

Swiss-Prot: P13647

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human CK-5 wasused as an immunogen.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells.The mitotically active basal layers of most stratified squamous epithelia express 10 to30% of their total protein as keratin. The two keratins specifically expressed in thesecells are the type II keratin CK-5 and its corresponding partner, type I keratin CK-14,both of which are essential for the formation of 8-nm filaments (1). CK-5 and calretininhave been useful in different studies as immunohistochemical markers suggestive ofmesothelioma, and their expression is analyzed for the histological differential diagnosiswith adenocarcinomas, especially when confronting with metastatic tumors of unknownorigin (2). Loss-of-function mutations in the keratin 5 gene (KRT5) affected familymembers and in six unrelated patients with Dowling-Degos disease (DDD), anautosomal dominant genodermatosis. This suggests a crucial role for keratins in theorganization of cell adhesion, melanosome uptake, organelle transport, and nuclearanchorage (3). Alternate Names: Anti-Cytokeratin-5 antibody, Anti-CK-5 antibody, Anti-Keratin-5 antibody, Anti-K5antibody, Anti-58 kDa cytokeratin antibody.

Spezifität: Species Reactivity: This antibody is reactive against Human.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:1000, Immunohistochemistry 1:50-100, Immunocytochemistry 1:50-100

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Signaltransduktion, Zytoskelett, Zytokeratine, Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

A. Western blot analysis on A431 cell lysate of using anti-CK-5 Rabbit Polyclonal, dilution 1:1000

B. Immunohistochemical analysis of paraffin-embedded human transitional carcinoma of bladder using anti-CK-5 Rabbit Polyclonal.