5-Methyl Cytosine (5-MeC) Antikörper

Antigen: 5-Methyl Cytosine (5-MeC)

Klonalität: Monoklonal (33D3)

Wirt: Maus

Reaktivität: Alle Spezies

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN255982

Produktbeschreibung

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 33D3

Beschreibung: Cytosine is a nucleobase whilst cytidine is a molecule (known as a nucleoside) that isformed when cytosine is attached to a ribose ring (also known as a ribofuranose) via aß,-N1-glycosidic bond. Recognizes the modified base 5-methylcytidine foundin DNA of plants and vertebrates. DNA methylation is a DNA modification process,which is involved in the control of gene expression. Reports suggest that in tumors, DNAis frequently globally hypomethylated compared to the DNA from normal tissue. Alternate Names: anti-5 MeCyd antibody, anti-5 Methyl Cytosine antibody, anti-5 MethylCytidine antibody,anti-5 MethylCytosine antibody, anti-5-MethylCytidine antibody, anti-5-MethylCytosineantibody.

Spezifität: Recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants andvertebrates. DNA methylation is a DNA modification process, which is involved in thecontrol of gene expression. Reports suggest that in tumors, DNA is frequently globallyhypomethylated compared to the DNA from normal tissue.Clone 33D3 has been developed to discriminate between the modified base 5-MeCydand the normal counterpart cytosine. This antibody has been reported for use inMethylated DNA immunoprecipitation (MeDIP). Species Reactivity: Reacts with Human, Rat and Mouse.

Anwendungen

Applikationshinweise: This product requires antigen retrieval using heat treatment priorto staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for thispurpose.

Konzentration: 1.0 mg/ml

Buffer: Phosphate buffered saline pH7.4. Preservative: 0.01% Thimerosal.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar