SLIT-ROBO rho GTPase Activating Protein 1 (SRGAP1) Antikörper

Antigen: SLIT-ROBO rho GTPase Activating Protein 1 (SRGAP1)

Synonyme: ARHGAP13, FLJ22166, KIAA1304, Arhgap13, 4930572H05Rik, RGD1566260, SRGAP1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN253121

Produktbeschreibung

Weitere Bezeichnung: ARHGAP13 / SRGAP1

Gen-ID: 57522

Swiss-Prot: NP_0658131

Immunogen: The epitope recognized by this antibody maps to a region between residue 979 and1029 of human SLIT-ROBO Rho GTPase activating protein 1 (Rho GTPase-activatingprotein 13) using the numbering given in entry NP_065813.1 (GeneID 57522).

Beschreibung: SRGAP1/ARHGAP13 is a member of the srGAP family of proteins that contain aRhoGAP domain, an SH3 domain, and a Fes/CIP4 homology domain and interact withthe intracellular domain of roundabout 1 (Robo1). Robo1 is the receptor for Slit signalingand is involved in neuronal cell migration and axon tract positioning.SRGAP1/ARHGAP13 functions to bind and decrease the level of active Cdc42 andRhoA small GTPases during Slit-Robo signaling. Alternate Names: anti-SRGAP1 antibody, anti-SRGAP-1 antibody, anti-SLIT-ROBO Rho GTPaseactivating protein 1 antibody, anti-ARHGAP13 antibody, anti-ARHGAP-13 antibody,anti-Rho GTPase-activating protein 13 antibody.

Spezifität: Species Reactivity: Cross reacts with Human.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunoprecipitation 2-5 ug/mg lysate,Western Blot 1:2000-1:10000

Konzentration: 0.2 mg/ml

Buffer: Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot and Immunoprecipitation: SRGAP1/ARHGAP13 Antibody [ABIN253121] - Detection of Human SRGAP1/ARHGAP13 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SRGAP1/ARHGAP13 antibody ABIN253121 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SRGAP1/ARHGAP13 was also immunoprecipitated by rabbit anti-SRGAP1/ARHGAP13 antibody ABIN253120, which recognizes an upstream epitope. Detection: Chemiluminescence ith exposure times of 30 seconds (A) and 1 second (B).