5'-3' Exoribonuclease 2 (XRN2) Antikörper

Antigen: 5'-3' Exoribonuclease 2 (XRN2)

Synonyme: zgc:55717, wu:fb38g12, wu:fj53d06, DKFZp459J063

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN252879

Produktbeschreibung

Weitere Bezeichnung: XRN2

Gen-ID: 22803

Swiss-Prot: NP_0363872

Immunogen: The epitope recognized by this antibody maps to a region between residue 900 and 950human 5'-3' exoribonuclease 2 using the numbering given in entry NP_036387.2(GeneID 22803).

Beschreibung: XRN2 is a 5'-to-3' exoribonuclease involved in 3'-end mRNA processing. XRN2 isimportant for transcription termination and is responsible for degrading the downstream3'-end-cleaved RNA. Recent studies show that XRN2 is recruited to 3'-end processingmachinery by p54nrb/PSF. Alternate names for XRN2 include 5'-3' exoribonuclease 2,DHM1-like protein, and DHP protein. Alternate Names: anti-XRN2 antibody, anti-XRN-2 antibody, anti-5'-3' exoribonuclease 2 antibody,anti-homolog of yeast Rat1 antibody, anti-homolog of mouse Dhm1-like protein antibody.

Spezifität: Species Reactivity: Cross reacts with Human.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunoprecipitation 2 to 5 mcg/mg,Western Blot 1:2000 to 1:10000

Buffer: Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot and Immunoprecipitation: XRN2 Antibody [ABIN252879] - Detection of Human XRN2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti- XRN2 antibody ABIN252879 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. XRN2 was also immunoprecipitated by another rabbit anti-XRN2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).