5'-3' Exoribonuclease 2 (XRN2) Antikörper

Antigen: 5'-3' Exoribonuclease 2 (XRN2)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN252877

Produktbeschreibung

Weitere Bezeichnung: XRN2

Gen-ID: 22803

Swiss-Prot: NP_0363872

Immunogen: The epitope recognized by this antibody maps to a region between residue 1 and 50 ofhuman 5'-3' exoribonuclease 2 using the numbering given in entry NP_036387.2(GeneID 22803).

Beschreibung: XRN2 is a 5'-to-3' exoribonuclease involved in 3'-end mRNA processing. XRN2 isimportant for transcription termination and is responsible for degrading the downstream3'-end-cleaved RNA. Recent studies show that XRN2 is recruited to 3'-end processingmachinery by p54nrb/PSF. Alternate names for XRN2 include 5'-3' exoribonuclease 2,DHM1-like protein, and DHP protein. Alternate Names: anti-XRN2 antibody, anti-XRN-2 antibody, anti-5'-3' exoribonuclease 2 antibody,anti-homolog of yeast Rat1 antibody, anti-homolog of mouse Dhm1-like protein antibody.

Spezifität: Species Reactivity: Cross reacts with Human and Mouse.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1:2000 to 1:10000

Buffer: Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blot and Immunoprecipitation: XRN2 Antibody [ABIN252877] - Detection of Human and Mouse XRN2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-XRN2 antibody ABIN252877 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B). XRN2 was immunoprecipitated by another rabbit anti-XRN2 antibodies and ABIN252879, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).