Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) Antikörper

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE)

Synonyme: IKKE, IKKI, IKK-i, KIAA0151, MGC125294, MGC125295, MGC125297, zgc:100844, IKBKE, MGC137373, MGC130907

Klonalität: Monoklonal (72B587)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN252485

Produktbeschreibung

Weitere Bezeichnung: IKBKE / IKKE

Gen-ID: 9641, 56489, 363984

Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acids175-188 of human IKKi/e protein. It will cross-react with mouse and rat IKKi/e and TBK1.

Klon: 72B587

Beschreibung: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factoror other cytokines results in phosphorylation and degradation of IkB releasing NF-kBdimers. NF-kB dimer subsequently translocates to the nucleus and activates targetgenes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkBkinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5).Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated(6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein hassignificant sequence homology with kinase domains of IKK1/a and IKK-2/b.Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa. Alternate Names: anti-I kappa B kinase epsilon antibody, anti-IkBKE antibody, anti-IKBKE proteinantibody, anti-IKK E antibody, anti-IKK i antibody, anti-IKK related kinase epsilonantibody, anti-IKKE antibody, anti-IKKI antibody, anti-Inducible I kappa B kinaseantibody, anti-inducible IkappaB kinase antibody, anti-Inhibitor of kappa light polypeptidegene enhancer in B cells kinase epsilon antibody, anti-Inhibitor of nuclear factor kappa Bkinase subunit epsilon antibody, anti-KIAA0151 antibody, anti-MGC125294 antibody,anti-MGC125295 antibody, anti-MGC125297 antibody.

Spezifität: In WB, a 80 kDa band is observed. Species Reactivity: Cross reacts with Human, Mouse and Rat.

Anwendungen

Applikationshinweise: Suggested working dilutions: Western Blot 1-3 ug/ml. Positive Controls: 293, Jurkat, or NIH 3T3

Konzentration: 0.5 mg/ml

Buffer: PBS. Preservative: 0.05 % Sodium Azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of IKKi using ABIN252485 at 2 ug/ml on 15 ugs of Jurkat cell lysate.