TBPL1 Antikörper (TBP-Like 1)

Details for Product anti-TBPL1 Antibody No. ABIN252470, Anbieter: Anmelden zum Anzeigen
Antigen
  • MGC:8389
  • MGC:9620
  • STUD
  • TLF
  • TLP
  • TRF2
  • tlf
  • 4732475G08
  • AW011832
  • AW491032
  • D18347
  • STD
  • TRP
  • Tlp
  • tlp
  • trf
  • trf2
  • trp
  • zgc:92340
  • TATA binding protein 1
  • TFIID-1
  • TRANSCRIPTION FACTOR IID-1
  • TATA-box binding protein like 1
  • TATA-binding protein 1
  • TATA-box binding protein like 1 L homeolog
  • TATA box binding protein-like 1
  • TBP-like 1
  • TATA binding protein 1
  • TBPL1
  • tbp1
  • tbpl1.L
  • tbpl1
  • Tbpl1
  • TBP1
Reaktivität
Human, Maus, Ratte (Rattus)
127
75
63
12
11
11
6
4
3
3
2
2
2
1
1
1
Wirt
Maus
59
49
19
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TBPL1 Antikörper ist unkonjugiert
8
8
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
100
55
49
38
37
34
19
18
17
13
6
3
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
Klon 4A794-15
Isotyp IgG1 kappa
Spezifität The recognizes distinct doublet bands of approximate size 66 kDa corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines.
Reinigung Protein G purified
Andere Bezeichnung TRF-2 (TBPL1 Antibody Abstract)
Hintergrund Gene Symbol: TERF2
Molekulargewicht Theoretical MW: 55.55 kDa
Gen-ID 7014
UniProt Q15554
Applikationshinweise Western Blot 2-4 μg/mL, Simple Western 1:50, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 0.1 μg/10^6 cells, ELISA 2 μg/mL, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 2 μg/10^6 cells, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 5 μg/mL, Proximity Ligation Assay, CyTOF-ready1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27366950)
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Immunoprecipitation (IP)/ Western Blot (WB) Analysis of TRF2 in HL60 cells: (Lane 1) ...
Simple Western (SimWes) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Simple Western: TRF-2 Antibody (4A794.15) [ABIN2524706] - Simple Western lane view sh...
Flow Cytometry (FACS) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Flow Cytometry: TRF-2 Antibody (4A794.15) [ABIN2524706] - Analysis using the Alexa Fl...
Flow Cytometry (FACS) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Flow Cytometry: TRF-2 Antibody (4A794.15) [ABIN2524706] - Analysis using the Alexa Fl...
Flow Cytometry (FACS) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Flow Cytometry: TRF-2 Antibody (4A794.15) [ABIN2524706] - Intracellular flow cytometr...
Western Blotting (WB) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Western Blot: TRF-2 Antibody (4A794.15) [ABIN2524706] - Analysis in human Jurkat cell...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [ABIN2524706] - Formalin-fix...
Immunofluorescence (IF) image for anti-TBP-Like 1 (TBPL1) antibody (ABIN252470) Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [ABIN2524706] - Sta...
Produkt verwendet in: Jahn, Rane, Paszkowski-Rogacz, Sayols, Bluhm, Han, Draškovič, Londoño-Vallejo, Kumar, Buchholz, Butter, Kappei: "ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator." in: EMBO reports, Vol. 18, Issue 6, pp. 929-946, 2017 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Human).

Saquilabon Cruz, Kong, Silva, Khatibzadeh, Thai, Berns, Yokomori: "Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites." in: Nucleic acids research, Vol. 44, Issue 3, pp. e27, 2016 (PubMed).

Altieri, Barisione, Lazzarini, Garuti, Bezante, Canepa, Spallarossa, Tocchetti, Bollini, Brunelli, Ameri: "Testosterone Antagonizes Doxorubicin-Induced Senescence of Cardiomyocytes." in: Journal of the American Heart Association, Vol. 5, Issue 1, 2016 (PubMed). Weitere Details: Western Blotting

Kim, Kim, Lee, Hong, Moon, Yoon, Kim, Kim, Kang, Kim, Choi, Goh, Kim, Lee, Ju, Jeong, Kim: "Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma." in: Theranostics, Vol. 6, Issue 3, pp. 357-68, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Konishi, Izumi, Shimizu: "TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends." in: The Journal of biological chemistry, Vol. 291, Issue 39, pp. 20798-810, 2016 (PubMed). Weitere Details: Immunocytochemistry,Immunoprecipitation,Immunofluorescence

Hasegawa, Okabe, Okamoto, Nakano, Shin-ya, Seimiya: "G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells." in: Biochemical and biophysical research communications, Vol. 471, Issue 1, pp. 75-81, 2016 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Shukla, Schmidt, Goldfarb, Cech, Parker: "Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects." in: Nature structural & molecular biology, Vol. 23, Issue 4, pp. 286-92, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB)

Picco, Coste, Giraud-Panis, Renno, Gilson, Pagès: "ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2." in: Oncotarget, Vol. 7, Issue 29, pp. 46615-46627, 2016 (PubMed). (Probematerial (Species): Human).

Fouquerel, Lormand, Bose, Lee, Kim, Li, Sobol, Freudenthal, Myong, Opresko: "Oxidative guanine base damage regulates human telomerase activity." in: Nature structural & molecular biology, Vol. 23, Issue 12, pp. 1092-1100, 2016 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Human).

Salvati, Rizzo, Iachettini, Zizza, Cingolani, DAngelo, Porru, Mondello, Aiello, Farsetti, Gilson, Leonetti, Biroccio: "A basal level of DNA damage and telomere deprotection increases the sensitivity of cancer cells to G-quadruplex interactive compounds." in: Nucleic acids research, Vol. 43, Issue 3, pp. 1759-69, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting,Chromatin Immunoprecipitation

Schertzer, Jouravleva, Perderiset, Dingli, Loew, Le Guen, Bardoni, de Villartay, Revy, Londoño-Vallejo: "Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA." in: Nucleic acids research, Vol. 43, Issue 3, pp. 1834-47, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Fujita, Yuno, Okuzaki, Ohki, Fujii: "Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123387, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Panneer Selvam, De Palma, Oaks, Oleinik, Peterson, Stahelin, Skordalakes, Ponnusamy, Garrett-Mayer, Smith, Ogretmen: "Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation." in: Science signaling, Vol. 8, Issue 381, pp. ra58, 2015 (PubMed).

Burla, Carcuro, Raffa, Galati, Raimondo, Rizzo, La Torre, Micheli, Ciapponi, Cenci, Cundari, Musio, Biroccio, Cacchione, Gatti, Saggio: "AKTIP/Ft1, a New Shelterin-Interacting Factor Required for Telomere Maintenance." in: PLoS genetics, Vol. 11, Issue 6, pp. e1005167, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

Clynes, Jelinska, Xella, Ayyub, Scott, Mitson, Taylor, Higgs, Gibbons: "Suppression of the alternative lengthening of telomere pathway by the chromatin remodelling factor ATRX." in: Nature communications, Vol. 6, pp. 7538, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Xi, Schmidt, Zaug, Ascarrunz, Cech: "A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression." in: Genome biology, Vol. 16, pp. 231, 2015 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Queisser, Heeg, Thaler, von Werder, Opitz: "Inhibition of telomerase induces alternative lengthening of telomeres during human esophageal carcinogenesis." in: Cancer genetics, Vol. 206, Issue 11, pp. 374-86, 2014 (PubMed). (Probematerial (Species): Human).

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." in: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (PubMed). (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Edwards, Orren, Machwe: "Strand exchange of telomeric DNA catalyzed by the Werner syndrome protein (WRN) is specifically stimulated by TRF2." in: Nucleic acids research, Vol. 42, Issue 12, pp. 7748-61, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

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