ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) Antikörper

Antigen: ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13)

Synonyme: Gm710, vWF-CP, ADAMTS13, TTP, VWFCP, C9orf8, FLJ42993, MGC118899, MGC118900, DKFZp434C2322, PRO4389, MGC126403, FLJ16363, FLJ32769, AI605170, ADAMTS-12, ADAMTS12

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: Immunpräzipitation (IP)

Produktnummer: ABIN251740

Produktbeschreibung

Weitere Bezeichnung: ADAMTS13

Gen-ID: 11093

Immunogen: Goat anti-ADAMTS13 affinity purified Antibodies were coupled to agarose beads using acyanogen bromide method.

Beschreibung: ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWFinto smaller forms in the serum. A thrombocytopenic disorder called thrombicthrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage ofvon Willebrands factor, and ADAMTS13 was identified as the enzyme responsible.ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a processstimulated by shear stress in the circulation and by binding to either platelet glycoproteinIBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathicform of TTR is found in patients with auto antibodies to ADAMTS13. The full lengthADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. Thevariants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen onWestern blots, between 110-190 kDa, perhaps indicating differentially processedADAMTS-13. Alternate Names: anti-ADAMTS13 antibody, anti-ADAMTS-13 antibody, anti-ADAM-TS13 antibody,anti-ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody,anti-VWFCP antibody, anti-vWF-CP antibody, anti-von Willebrand factor-cleavingprotease antibody.

Spezifität: Species Reactivity: Reacts with Human.

Anwendungen

Applikationshinweise: Immunoprecipitation 30 to 50 ul of gel slurry per 100 ul of human serum.

Buffer: 0.01 M Phosphate, pH 7.2, 0.1 M NaCl. Preservative: 0.09% Sodium Azide.

Lagerung: Store at 4C. Do not freeze.

Forschungsgebiet: Proteolyse / Ubiquitin, Metalloprotease, Extrazelluläre Matrix, Gerinnung

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of Human ADAMTS13 by Western Blot and Immunoprecipitation. Samples: Pooled normal human serum (300 mcl/IP, 10% of IP loaded, 1.5 mcl for input). Antibodies: Agarose immobilized goat anti-ADAMTS13 antibodies ABIN251737, ABIN251738, ABIN251739, ABIN251740 and ABIN251741 were used at the equivalent of 35 mcg of antibody per IP. Detection: Chemiluminescence with an exposure time of 30 seconds.