4-Amino Biphenyl DNA Antikörper

Antigen: 4-Amino Biphenyl DNA

Klonalität: Monoklonal (4C11)

Wirt: Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Immunfluoreszenz (IF)

Produktnummer: ABIN251623

Produktbeschreibung

Immunogen: 4-amino Biphenyl DNA.

Klon: 4C11

Beschreibung: 4-amino Biphenyl (4-ABP) is an aromatic amine (arylamine). 4-ABP can be found as aconstituent of tobacco smoke, in the form of an environmental carcinogen. It ismetabolized by hepatic cytochrome P4501A2 to yield a direct-acting mutagen known asN-hydroxy-aminobiphenyl. Yet, the accumulation of biologically active metabolites isdependent on the activation and detoxification of N-acetylation. 4-ABP has been shown to be a bladder carcinogen in humans and rodents. As well asgenetic factors, 4-ABP may also play a role in breast cancer. It is hypothesized thatsmokers may have higher levels of DNA adducts than non-smokers and that the role of4-ABP-DNA adducts and their relationship to tobacco exposure may be related to breasttumorigenesis. The antibody has been used to investigate the role of 4-ABP in thestudy of human breast cancer by measuring 4-ABP-DNA adducts. Alternate Names: anti-4-amino-Biphenyl DNA antibody, 4-AMBP DNA antibody, 4-aminobiphenyl DNAantibody.

Spezifität: This antibody is specific for 4-amino Biphenyl DNA. Species Reactivity: All DNAs. Localization: Nuclear.

Anwendungen

Applikationshinweise: This antibody has specifically been used in ELISA. This clone has been shown to workin immunocytochemistry, immunofluorescence, and immunohistochemistry, on bothparaffin-embedded and frozen sections. Other applications have not been tested.Suggested working dilutions: *ELISA - (+)Immunohisto/cytochemistry - 1:50-1:100

Konzentration: 1.4 mg/ml

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Forschungsgebiet: DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: al-Atrash, Zhang, Lin et al.: "Quantitative immunohistochemical analysis of 4-aminobiphenyl-DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis." in: Chemical research in toxicology, Vol. 8, Issue 5, pp. 747-52, 1995 (PubMed).

McQueen, Chau: "Neonatal ontogeny of murine arylamine N-acetyltransferases: implications for arylamine genotoxicity." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 73, Issue 2, pp. 279-86, 2003 (PubMed).

Faraglia, Chen, Gammon et al.: "Evaluation of 4-aminobiphenyl-DNA adducts in human breast cancer: the influence of tobacco smoke." in: Carcinogenesis, Vol. 24, Issue 4, pp. 719-25, 2003 (PubMed).