LC3B Antikörper

Antigen: LC3B

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus

Applikation: Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN250723

Produktbeschreibung

Gen-ID: 81631

Immunogen: A synthetic peptide made to an internal portion of the human LC3 protein sequence(between residues 50-150).

Beschreibung: Autophagy is a process of intracellular bulk degradation in which cytoplasmiccomponents, including organelles, are sequestered within double-membrane vesiclesthat deliver the contents to the lysosome/vacuole for degradation. Duringmacroautophagy, the sequestering vesicles, termed autophagosomes, fuse with thelysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) intothe lumen of the degradative compartment. There are 16 proteins participating in theautophagy pathway in human. The autophagy protein LC3, a mammalian homologue ofAtg8, was originally identified as microtubule-associated protein 1 light chain 3. It is acomponent of both the MAP1A and MAP1B microtubule-binding domains and theheavy-chain independent regulation of LC3 expression might modify MAP1microtubule-binding activity during development. LC3 is the only known mammalianprotein identified that stably associates with the autophagosome membranes. LC3-I iscytosolic and LC3-II is membrane bound and enriched in the autophagic vacuolefraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker fordistinguishing autophagy in mammalian cells. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 Bantibody, anti-MAP1A/b light chain 3 B antibody, anti-Autophagy-related protein LC3 Bantibody, anti-Autophagy-related ubiquitin-like modifier LC3 B antibody.

Spezifität: Species Reactivity: Reacts with human, mouse, rat, xenopus and bovine. Localization: LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunohistochemistry 1:200-1:400,immunoprecipitation 20ug/500ug of protein,Western Blot 2 ug/ml,Immunohistochemistry-Paraffin . Positive Controls: Human and mouse brain lysate

Konzentration: 1.12 mg/ml

Buffer: PBS, 30% glycerol

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Krebs, Autophagie, Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemistry: LC3 Antibody [ABIN250719SS] - Staining of human brain, cerebral cortex, cell processes in gray matter using ABIN250719.

Western Blot: LC3 Antibody [ABIN250719SS] Detection of autophagic LC3 in mouse ES cell lysate using ABIN250719. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of ABIN250719, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center

Publikationen

Publikationen: Kabeya, Mizushima, Ueno et al.: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Mizushima, Yamamoto, Hatano et al.: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).

Zhang, Yu, Pan et al.: "Small molecule regulators of autophagy identified by an image-based high-throughput screen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 48, pp. 19023-8, 2007 (PubMed).

Lee, Cao, Mostoslavsky et al.: "A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3374-9, 2008 (PubMed).

Swerdlow, McColl, Rong et al.: "Apoptosis inhibition by Bcl-2 gives way to autophagy in glucocorticoid-treated lymphocytes." in: Autophagy, Vol. 4, Issue 5, pp. 612-20, 2008 (PubMed).

Lee, Finkel: "Regulation of autophagy by the p300 acetyltransferase." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6322-8, 2009 (PubMed).