RAD51-Like Protein 3 (RAD51L3) Antikörper

Antigen: RAD51-Like Protein 3 (RAD51L3)

Synonyme: Trad, R51H3, HsTRAD, RAD51D

Klonalität: Monoklonal (5B3-6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN250499

Produktbeschreibung

Weitere Bezeichnung: RAD51L3

Gen-ID: 5892

Immunogen: His-tagged hRAD51D, overexpressed in E. coli.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 5B3-6

Beschreibung: Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B,RAD51C, and RAD51D. Each of these homologues interacts with one or more of theothers, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C (1). RAD51D's expression pattern andsequence similarity to other RAD51 family members most likely make it part of acomplex of proteins required for DNA repair and meiotic recombination. Alternate Names: anti-RAD51L3 antibody, anti-RAD51-like 3 (S. cerevisiae) antibody, anti-TRAD antibody,anti-R51H3 antibody, anti-HsTRAD antibody, anti-RAD51D antibody, anti-recombinationrepair protein antibody, anti-DNA repair protein RAD51 homolog 4 antibody.

Spezifität: ABIN151303 is specific for Rad51D. Species Reactivity: ABIN151303 recognizes human Rad51D. It does not work on mouse. Other specieshave not been tested. ABIN151303 does not cross-react with Rad51B, Rad51C, Rad51,XRCC2, or XRCC3 in Western analysis.

Anwendungen

Applikationshinweise: By Western blot, this antibody recognizes a band at ~40 kDa, representing Rad51Dprotein.Suggested working dilutions: *Western Blot - 1:1,000, Immunofluorescence - 1:500, Immunoprecipitation - ND

Lagerung: Store at 4C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Rad51D detected in HEK293 lysates using a 1:1,000 dilution of ABIN151303 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School

Publikationen

Publikationen: Pittman, Weinberg, Schimenti: "Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene." in: Genomics, Vol. 49, Issue 1, pp. 103-11, 1998 (PubMed).

Kawabata, Saeki: "Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family." in: Biochemical and biophysical research communications, Vol. 257, Issue 1, pp. 156-62, 1999 (PubMed).

Masson, Tarsounas, Stasiak et al.: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).

Maacke, Hundertmark, Miska et al.: "Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen." in: Journal of cancer research and clinical oncology, Vol. 128, Issue 4, pp. 219-22, 2002 (PubMed).

Lambert, Lopez: "Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells." in: Oncogene, Vol. 21, Issue 25, pp. 4065-9, 2002 (PubMed).

Fan, Kumaravel, Jalali et al.: "Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression." in: Cancer research, Vol. 64, Issue 23, pp. 8526-33, 2004 (PubMed).

Hinz, Tebbs, Wilson et al.: "Repression of mutagenesis by Rad51D-mediated homologous recombination." in: Nucleic acids research, Vol. 34, Issue 5, pp. 1358-68, 2006 (PubMed).

Shammas, Shmookler Reis, Koley et al.: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed).